EasySep™ Direct HLA Crossmatch T Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen or lymph node kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Direct HLA Crossmatch T Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen or lymph node kit

From: 535 USD
Catalog #
19671_C
Immunomagnetic negative selection from whole blood, spleen or lymph node kit

Product Advantages


  • > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis

  • Up to 99.9% purity of isolated cells

  • Fast, easy-to-use, and column-free

  • Isolated cells are untouched

What's Included

  • EasySep™ Direct HLA Crossmatch T Cell Isolation Kit (Catalog #19671)
    • EasySep™ Direct HLA Crossmatch T Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
  • EasySep™ Direct HLA Crossmatch T Cell Isolation Kit for RoboSep™ (Catalog #19671RF)
    • EasySep™ Direct HLA Crossmatch T Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Direct HLA Crossmatch T Cell Isolation Kit can be used to isolate highly purified T cells directly from human whole blood, spleen or lymph nodes by immunomagnetic negative selection. This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry crossmatch.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
Lymph Node, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HLA, Immunology

Data Figures

Starting with human whole blood from normal healthy donors, the T cell (CD3+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.9% (gated on CD45). In the above example, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 38.0% and 93.2% (gated on CD45), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19671
Lot #
18F92320 or higher
Language
English
Catalog #
19671RF
Lot #
18F92320 or higher
Language
English
Document Type
Safety Data Sheet
Catalog #
19671
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
19671RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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