Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts

Polystyrene plate with lid and polyester membrane inserts
Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts

Polystyrene plate with lid and polyester membrane inserts for cell culture that feed basolaterally

48 Inserts
Catalog # 38023
315 USD
Required Products
  1. PneumaCult™-ALI Medium
    PneumaCult™-ALI Medium

    Serum- and BPE-free medium for human airway epithelial cells cultured at the air-liquid interface

  2. PneumaCult™-Ex Medium
    PneumaCult™-Ex Medium

    Serum- and BPE-free medium for expansion of primary human airway epithelial cells

  3. PneumaCult™-Ex Plus Medium
    PneumaCult™-Ex Plus Medium

    Serum- and BPE-free medium for expansion of primary human airway epithelial cells

Overview

Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts support the growth of both anchorage-dependent and anchorage-independent cells, including those that are fed at the basolateral surface or in co-culture systems. These permeable inserts are recommended for culturing airway epithelial cells at the air-liquid interface, and are available in a kit (Catalog #05021) that includes PneumaCult™-ALI Medium (Catalog #05001).

48 Inserts; 4 x 12-well plates, each with 12 inserts
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Cell Culture

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Costar® 12 mm Transwell®, 0.4 µm Pore Polyester Membrane Inserts
Catalog #
38023
Lot #
All
Language
English

Educational Materials (3)

Brochure
Modeling Cystic Fibrosis Airway: PneumaCult™-Ex Plus and PneumaCult™-ALI
Brochure
PneumaCult™ Culture Media for Human Airway Epithelial Cells
Webinar
Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
51:30
Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells

Data and Publications

Data

Figure 1. Representative Images of ALI Culture Morphology

A side-by-side comparison was performed between Transwell® Inserts and competitor inserts of the same material and pore size. HBECs were seeded onto the inserts at passage 3 (P3) and differentiated in PneumaCult™-ALI for 21 days. Air-liquid interface (ALI) cultures generated using PneumaCult™ and Transwell® Inserts provided the most optimal morphology.

Figure 2. Differential Epithelial Cell Marker Expression in ALI Cultures Generated with Different Inserts

HBECs were seeded onto the inserts at P3 and differentiated in PneumaCult™-ALI for 21 days. Gene expression of goblet (MUC5B) and ciliated (FOXJ1) cell markers was assessed by qPCR and normalized to beta actin. Comparison was made with competitor inserts of the same material and pore size. Higher expression of goblet and ciliated cell markers was observed when using PneumaCult™ together with Transwell® inserts.

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