Caspase-3/7 Activity Plate Reader Assay Kit, Blue
For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis
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Overview
Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). When added to apoptotic cells, Ac-DEVD-AMC is cleaved by activated caspase-3/7, generating a blue fluorescent signal that can be detected by standard plate readers.
Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases—caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase assays are useful for quantifying caspase-3/7 activity in apoptotic cells and for screening caspase-3/7 inhibitors.
Data Figures

Figure 1. Caspase-3/7 Activity Plate Reader Assay Kit, Blue
Jurkat cells were treated with 0.2% DMSO (Control) or 1 µM staurosporine (STSP), and in the presence or absence of Z-VAD-FMK (a pan-caspase inhibitor). Caspase-3/7 activity was assessed as a measure of fluorescence using the Caspase-3/7 Activity Plate Reader Assay Kit, Blue. Data are shown as the mean from two independent experiments.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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