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PancreaCult™

Complete, Defined Medium for Pancreatic Organoid Culture

PancreaCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishing and maintaining mouse pancreatic exocrine organoids. Derived from progenitor cells residing in pancreatic ducts, these "mini-pancreata" provide an in vitro, organotypic culture system for studying pancreatic cell biology, disease, and cancer. Organoids grown in PancreaCult™ feature an epithelium expressing genes marking pancreatic stem cells (LGR5), progenitor cells (PDX1, SOX9), and ductal cells (CAR2, MUC1, KRT19). Pancreatic organoids can be passaged every 3 - 6 days for long-term maintenance and can also be cryopreserved.

Researcher Interview: Dr. Meritxell Huch

In 2013, Dr. Meritxell Huch et al. first described the culture of pancreatic tissues as organoids. In this researcher interview, Dr. Huch discusses what led her to studying organoids and provides her insight for where she thinks the use of organoids is headed.

Why Use PancreaCult™?

  • In vitro system for generating organoids within a week.
  • No injury models, hand-picking of ducts or cell sorting required.
  • Serum-free and defined medium formulation.
  • Organoids can be grown from duct fragments or single cells and be cultured in matrix domes or suspension.

Products for Pancreatic Organoid Culture

PancreaCult™ Organoid Growth Medium

Recommended for:

Establishment and maintenance of hepatic stem cells in Corning® Matrigel® domes or in a dilute Matrigel® suspension

Species:

Mouse

Components:

  • PancreaCult™ OGM Mouse Basal Medium, 95 mL
  • PancreaCult™ OGM Mouse Supplement, 5 mL

Resources:

Download our Technical Bulletin

Mouse Pancreatic Organoids

Recommended for:

Convenient establishment and standardization of pancreatic organoid cultures in the laboratory

Species:

Mouse

Contents:

Frozen mouse pancreatic exocrine organoid segments

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Brand History

Despite the prevalence and importance of research into pancreatic cancer during the recent decades, ex vivo culture of pancreatic exocrine tissues has remained difficult until recently. In 2013, Dr. Meritxell Huch et al. described an efficient method for the propagation of pancreatic tissues in vitro as organoids. In 2014, Dr. Hans Clevers and Foundation Hubrecht Organoid Technology signed an agreement with STEMCELL Technologies to manufacture and distribute cell culture media for organoids. PancreaCult™ Organoid Growth Medium (Mouse) was released in early 2018, providing researchers with a robust medium for establishing and maintaining pancreatic exocrine organoids.


Key Applications of Pancreatic Organoids

Cancer Research

Avars M, et al. (2017) IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth. Oncotarget. 8(48): 83370-83383.

Ferreira RMM, et al. (2017) Duct- and acinar-derived pancreatic ductal adenocarcinomas show distinct tumor progression and marker expression. Cell Rep. 21(4): 966-978.

Chio II, et al. (2016) NRF2 Promotes Tumor Maintenance by Modulating mRNA Translation in Pancreatic Cancer. Cell. 166(4): 963-976.

Baker LA, et al. (2016) Modeling pancreatic cancer with organoids. Trends Cancer. 2(4): 176-190.

David CJ, et al. (2016) TGF-β tumor suppression through a lethal EMT. Cell. 164(5): 1015-30.

Boj SF, et al. (2015) Organoid models of human and mouse ductal pancreatic cancer. Cell. 160(1-2): 324-38.

Roe JS, et al. (2017) Enhancer reprogramming promotes pancreatic cancer metastasis. Cell. 170(5): 875-888.

Sinha S, et al. (2017) PanIN neuroendocrine cells promote tumorigenesis via neuronal cross-talk. Cancer Res. 77(8): 1868-1879.

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