Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14

Rat monoclonal IgG2a antibody against mouse CD62L (L-selectin)
From: 55 USD

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Rat monoclonal IgG2a antibody against mouse CD62L (L-selectin)
From: 55 USD

Overview

The MEL-14 antibody reacts with murine CD62L (L-Selectin or LECAM-1), an ~74 - 95 kDa type I glycoprotein expressed on the surface of a majority of leukocytes. CD62L mediates leukocyte-endothelial cell interactions through its association with ligands such as CD34, glyCAM-1, MAdCAM-1 and PSGL-1. It functions to facilitate lymphocyte rolling on the vascular endothelium, trafficking of lymphocytes to the lymph nodes, and homing of lymphocytes and neutrophils to sites of inflammation. Murine CD62L is expressed on most thymocytes, the highest expression levels being found on subsets of immunocompetent or dividing progenitor cells, as well as on neutrophils, eosinophils, monocytes, and subsets of B, T and NK cells. The MEL-14 antibody may be used together with antibodies to other cell surface markers to distinguish naïve, memory and effector T cells. The MEL-14 antibody has been shown to block migration of lymphocytes to lymph nodes and to inhibit leukocyte rolling.
Subtype:
Primary Antibodies
Target Antigen:
CD62L (L-selectin)
Alternative Names:
CD62 ligand, L-selectin, LAM-1, LECAM-1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 1, Ly-22, MEL-14, Pln homing receptor, PLNHR
Reactive Species:
Mouse
Conjugation:
APC; Biotin; FITC; PE; Unconjugated; Alexa Fluor 488; PerCP-Cyanine5.5
Host Species:
Rat
Cell Type:
T Cells
Application:
Flow Cytometry; Functional Assay; Immunofluorescence; Immunocytochemistry; Immunohistochemistry; Immunoprecipitation; CyTOF
Area of Interest:
Immunology
Clone:
MEL-14
Gene ID:
20343
Isotype:
IgG2a, kappa

Scientific Resources

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow Cytometry Analysis of C57BL/6 Mouse Splenocytes

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of mouse splenocytes (gated on CD4+ cells) processed with EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit and labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PE and an anti-mouse CD44 antibody, FITC. Labeling of the Start splenocytes prior to cell isolation is shown (C).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD62L (L-Selectin) Antibody, Clone MEL-14, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.