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Anti-Mouse CD19 Antibody, Clone 1D3

Rat monoclonal IgG2a antibody against mouse CD19

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Anti-Mouse CD19 Antibody, Clone 1D3

Rat monoclonal IgG2a antibody against mouse CD19

From: 65 USD
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Rat monoclonal IgG2a antibody against mouse CD19
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Overview

The 1D3 antibody reacts with mouse CD19, an ~95 kDa type 1 transmembrane glycoprotein expressed on the surface of B cells throughout all stages of development, from early pre-B cells to plasma cells. Expression is down-regulated but persists in terminally differentiated plasma cells. CD19 is also found on follicular dendritic cells. By associating with CD21 and CD81, CD19 functions as a co-receptor for the B cell receptor and is involved in B cell activation and differentiation. Activation of CD19 is accompanied by phosphorylation of the cytoplasmic domain, which promotes binding to kinases and the induction of intracellular signaling cascades. Mutations in CD19 can result in severe immunodeficiency syndromes. Clone 1D3 recognizes the same epitope as clone 6D5 in cross-competition assays.
Subtype
Primary Antibodies
Target Antigen
CD19
Alternative Names
B4
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
B Cells
Species
Mouse
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
1D3
Gene ID
12478
Isotype
IgG2a, kappa

Data Figures

Data for unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or a rat IgG2a, kappa isotype control antibody followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, APC (filled histogram) or a rat IgG2a, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a biotinylated rat IgG2a, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, FITC (filled histogram) or a rat IgG2a, kappa isotype control antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, PE (filled histogram) or a rat IgG2a, kappa isotype control antibody, PE (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, APC
Catalog #
60112AZ.1, 60112AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, Biotin
Catalog #
60112BT.2, 60112BT, 60112BT.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3
Catalog #
60112.1, 60112
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, FITC
Catalog #
60112FI.2, 60112FI, 60112FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5
Catalog #
60112PS, 60112PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PE
Catalog #
60112PE.1, 60112PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, APC
Catalog #
60112AZ.1, 60112AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, Biotin
Catalog #
60112BT.2, 60112BT, 60112BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3
Catalog #
60112.1, 60112
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, FITC
Catalog #
60112FI.2, 60112FI, 60112FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PerCP-Cy5.5
Catalog #
60112PS, 60112PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD19 Antibody, Clone 1D3, PE
Catalog #
60112PE.1, 60112PE
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (2)

Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research
Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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