Anti-Human CD83 Antibody, Clone HB15e

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83
From: 90 USD

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83
From: 90 USD

Overview

The HB15e antibody reacts with CD83, an ~45 kDa type I transmembrane glycoprotein and member of the Siglec protein family, containing an extracellular V-type Ig-like domain and a C-terminal cytoplasmic tail. A soluble form of CD83 has also been identified. CD83 is expressed predominantly on circulating and tissue dendritic cells, including Langerhan cells and interdigitating reticulum cells. It is expressed weakly on activated lymphocytes and may also be found on neutrophils, monocytes and macrophages. Expression of CD83 is typically transient, except on dendritic cells, and correlates with upregulation of HLA class II antigen. Its function, though ill-defined, is essential for the development and maintenance of the immune system and evidence suggests it may be involved in cell adhesion and signaling, antigen presentation, and lymphocyte activation. The soluble form of CD83 appears to be immunosuppressive. The epitope on CD83 bound by the HB15e antibody reportedly overlaps with that of antibody 1E11.
Subtype:
Primary Antibodies
Target Antigen:
CD83
Alternative Names:
B-cell activation protein, BL11, HB15
Reactive Species:
Human; Rhesus; Cynomolgus; Baboon; Chimpanzee; Macaque
Conjugation:
APC; Biotin; FITC; PE; Unconjugated; Alexa Fluor 488
Host Species:
Mouse
Cell Type:
Dendritic Cells
Application:
Flow Cytometry; Immunohistochemistry; Immunoblotting
Area of Interest:
Immunology
Clone:
HB15e
Gene ID:
9308
Isotype:
IgG1, kappa

Scientific Resources

Educational Materials

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Product Applications

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Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, followed by a rat anti-mouse IgG1 antibody, PE (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, APC (filled histogram) or a mouse IgG1, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, Biotin followed by streptavidin (SAV) APC (filled histogram), or a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Anti-Human CD83 Antibody, Clone HB15e, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, PE (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.