This monoclonal antibody (clone 2D10.4) reacts with human cluster of differentiation 80 (CD80), also known as B7-1, a 60 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed by activated B cells, T cells, macrophages, and dendritic cells. Use this recombinant monoclonal antibody in applications requiring detection of CD80, such as flow cytometry (FC). This antibody clone has been verified for labeling CD80+ primary human M1 macrophages grown in ImmunoCult™-SF Macrophage Medium (Catalog #10961) containing Human Recombinant M-CSF (Catalog #78057), and then polarized with Human Recombinant IFN-gamma (Catalog #78020) and Lipopolysaccharide from E. coli (O55:B5) (Catalog #100-1270).
CD80 has a high affinity for binding to two T cell receptors; CD28 and CD152 (also known as CTLA-4). The interaction of CD80 with CD28 provides a potent co-stimulatory signal for T cell activation through the CD3 complex, while its interaction with CD152 provides an inhibitory signal for T cell activation. CD80 together with CD86, another ligand of CD28 also known as B7-2, plays a major role in regulation of T cell activation. CD80 dysfunction is associated with diseases such as squamous cell carcinoma of gallbladder (SCC) and myocarditis. This antibody can also be used as a marker to assess classically activated type 1 (M1) macrophages.