Anti-Human CD4, Clone RPA-T4

Mouse monoclonal IgG1 antibody against human, chimpanzee CD4
Catalog #
100-0298_C
Mouse monoclonal IgG1 antibody against human, chimpanzee CD4
From: 41 USD

Overview

The RPA-T4 antibody reacts with CD4, an ~59 kDa single-chain type 1 transmembrane glycoprotein and member of the immunoglobulin (Ig) superfamily; CD4 contains four extracellular Ig-like domains (D1 - D4). The epitope for RPA-T4 has been localized to the D1 domain of the protein, which has a structure resembling an Ig variable domain. CD4 is expressed at relatively high levels by most thymocytes and a subpopulation of T cells (T helper cells), and at lower levels by peripheral blood monocytes and macrophages. CD4 binds to a nonpolymorphic region of MHC II and acts as a co-receptor to the T cell receptor (TCR) in MHC II-restricted antigen recognition by enhancing the affinity of the association between the TCR and MHC II-antigen complex. CD4 also functions to amplify signals from the TCR to the cytoplasm through the interaction of its intracellular domain with cytoplasmic tyrosine kinases, such as Lck. Moreover, CD4 is a receptor for the human immunodeficiency virus (HIV). Binding of the RPA-T4 antibody blocks HIV binding and mixed lymphocyte reaction (MLR), and inhibits the activation of CD4+ T cells in vitro by interfering with the association of CD4 and HLA-DR. It also partially blocks the binding of antibody clone SK3. The epitope of the RPA-T4 antibody is reportedly sensitive to formaldehyde fixation.
Subtype
Primary Antibodies
Target Antigen
CD4
Alternative Names
Leu3, L3T4, T4
Reactive Species
Chimpanzee, Human
Conjugation
APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
T Cells
Application
CyTOF, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
RPA-T4
Gene ID
920
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD4, Clone RPA-T4, APC
Catalog #
100-0298, 100-0299, 100-0300
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4, Clone RPA-T4, FITC
Catalog #
100-0301, 100-0302, 100-0303
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4, Clone RPA-T4, PE
Catalog #
100-0304, 100-0305, 100-0306
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD4, Clone RPA-T4
Catalog #
100-0307
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4, Clone RPA-T4, APC
Catalog #
100-0298, 100-0299, 100-0300
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4, Clone RPA-T4, FITC
Catalog #
100-0301, 100-0302, 100-0303
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4, Clone RPA-T4, PE
Catalog #
100-0304, 100-0305, 100-0306
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD4, Clone RPA-T4
Catalog #
100-0307
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, APC or a mouse IgG1, kappa APC isotype control antibody.

Figure 1. Data for Anti-Human CD4 Antibody, Clone RPA-T4, APC

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, FITC or a mouse IgG1, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Human CD4 Antibody, Clone RPA-T4, FITC

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, PE or a mouse IgG1, kappa PE isotype control antibody.

Figure 3. Data for Anti-Human CD4 Antibody, Clone RPA-T4, PE

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone RPA-T4, followed by a goat anti-mouse IgG1 antibody, PE or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG1 antibody, PE.

Figure 4. Data for Anti-Human CD4 Antibody, Clone RPA-T4, Unconjugated

Flow cytometry analysis of human PBMCs labeled with Anti-Human CD4 Antibody, Clone RPA-T4, followed by a goat anti-mouse IgG1 antibody, PE (filled histogram) or a mouse IgG1, kappa isotype control antibody, followed by a goat anti-mouse IgG1 antibody, PE (solid line histogram).

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.