Human Peripheral Blood CD19+CD27- Naïve B Cells (Donor-Matched), Frozen

Primary human cells, frozen

Human Peripheral Blood CD19+CD27- Naïve B Cells (Donor-Matched), Frozen

Primary human cells, frozen

From: 1,605 USD
Catalog #
200-0321_C
Primary human cells, frozen
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Overview

Obtain ready-to-use, ethically sourced, donor-matched primary human CD19+CD27- naïve B cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications.

CD19+CD27+ memory B cells (Catalog #200-0320; purchased separately) are isolated from peripheral blood mononuclear cells using positive immunomagnetic separation, and donor-matched CD19+CD27- naïve B cells are subsequently isolated using negative immunomagnetic separation. Cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation, high-resolution HLA typing (Class I and Class II alleles), and CMV status are available upon request. Acid-citrate-dextrose solution A (ACDA) is used as an anticoagulant during collection.

Request custom services in the comment box above. These include alternative formats (size, vials, anti-coagulant), product characterizations (HLA status, blood type, genotype, phenotype, etc.), donor specifications (e.g. BMI category, smoking status, ethnicity, etc.), and additional viral screening (CMV, EBV, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. A summary of product information is available on the Certificate of Analysis.

Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.

Note: If you only require CD19+CD27- naïve B cells, and not CD19+CD27+ memory B cells with donor-matched CD19+CD27- naïve B cells, we recommend using Human Peripheral Blood CD19+CD27- Naïve B Cells, Frozen (Catalog #70032).
Subtype
Frozen
Cell Type
B Cells
Species
Human
Cell and Tissue Source
Peripheral Blood
Purity
Memory B Cells are ≥ 85% CD19+CD27+ by flow cytometry, Naive B Cells are ≥ 85% CD19+CD27- by flow cytometry

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications