Human Peripheral Blood CD19+CD27- Naïve B Cells (Donor-Matched), Frozen
Primary human cells, frozen
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Overview
CD19+CD27+ memory B cells (Catalog #200-0320; purchased separately) are isolated from peripheral blood mononuclear cells using positive immunomagnetic separation, and donor-matched CD19+CD27- naïve B cells are subsequently isolated using negative immunomagnetic separation. Cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation, high-resolution HLA typing (Class I and Class II alleles), and CMV status are available upon request. Acid-citrate-dextrose solution A (ACDA) is used as an anticoagulant during collection.
Request custom services in the comment box above. These include alternative formats (size, vials, anti-coagulant), product characterizations (HLA status, blood type, genotype, phenotype, etc.), donor specifications (e.g. BMI category, smoking status, ethnicity, etc.), and additional viral screening (CMV, EBV, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. A summary of product information is available on the Certificate of Analysis.
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Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Note: If you only require CD19+CD27- naïve B cells, and not CD19+CD27+ memory B cells with donor-matched CD19+CD27- naïve B cells, we recommend using Human Peripheral Blood CD19+CD27- Naïve B Cells, Frozen (Catalog #70032).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
