On-Demand Human Intestinal Organoid Course

Virtual training on culturing primary human intestinal organoids

Welcome to the On-Demand Human Intestinal Organoid Course. In this self-paced, virtual course, you will learn how to grow primary tissue-derived human intestinal organoids through a series of lectures, technical lab videos, and assessments.

Before You Begin

The training curriculum is designed to be reviewed sequentially. We encourage you to watch each session before attempting that part of the protocol. Ensure that you have all the required materials before beginning the protocol.

When you're ready, start with Session 1.

Required Materials

Use our Interactive Product Finder to determine which products are best for your intestinal experiments, and generate a complete materials list for culturing human intestinal organoids.

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Instructor Bios

Dr. Kiran Bhullar, Scientist, Scientific Support, Epithelial Cell Biology

Dr. Martin Stahl, Senior Scientist, Intestinal


Course Curriculum

Lecture 1: Introduction to Intestinal Biology

  • Introduction to Intestinal Biology
  • Introduction to Intestinal Organoids
  • Intestinal Model and Culture Systems

Mini Quiz: Test Your Knowledge


Estimated time for completion: 30 minutes

Lecture 2: Introduction to IntestiCult™ Human Products and Workflows

  • Introduction to Human IntestiCult™ Products
  • Introduction to Organoid Workflows

Mini Quiz: Test Your Knowledge


Estimated time for completion: 25 minutes

Lecture 3: Human Intestinal Organoid Morphology

  • Morphological Assessment for Primary Initiation Cultures
  • Morphological Assessment of Human Intestinal Organoids (Passaging)

Mini Quiz: Test Your Knowledge


Estimated time for completion: 20 minutes

Lecture 4: Application of Human Intestinal Organoids

  • Characterization and Function of Intestinal Organoid Cultures
  • Application Highlights

Mini Quiz: Test Your Knowledge


Estimated time for completion: 45 minutes

Lecture 5: Human Intestinal Organoids and Organs-on-Chips

  • The “Why” of Organ-on-a-Chip
  • How to Choose an Intestine Chip

Estimated time for completion: 30 minutes

Complete this final assessment to test your knowledge and receive a certificate following successful completion.


Estimated time for completion: 15 minutes

Additional Resources

Explore frequently asked questions, protocols, and additional resources for culturing human intestinal organoids.

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