Human Peripheral Blood-Derived Mature Dendritic Cells, ACF-Cultured, Frozen

Primary human cells, frozen

Human Peripheral Blood-Derived Mature Dendritic Cells, ACF-Cultured, Frozen

Primary human cells, frozen

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Primary human cells, frozen
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Streamline your assays with ready-to-use, ethically sourced, animal component-free-cultured, primary human mature dendritic cells (DCs). With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need.

Peripheral blood (PB) monocytes are isolated by negative immunomagnetic selection and cultured for 5 days in serum- and animal component-free ImmunoCult™-ACF Dendritic Cell Medium (Catalog #10986) and ImmunoCult™-ACF Dendritic Cell Differentiation Supplement (Catalog #10988), which contains a combination of recombinant human cytokines formulated to support the differentiation of human monocytes to immature DCs (iDCs). Next, the iDCs are matured for 2 days with ImmunoCult™ Dendritic Cell Maturation Supplement (Catalog #10989).

Cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

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Browse our Frequently Asked Questions (FAQs) on Primary Cells.
CryoStor® CS10
Cell Type
Dendritic Cells
Cell and Tissue Source
Peripheral Blood
Area of Interest
Disease Modeling, Stem Cell Biology, Cell Therapy Development
Donor Status
≥ 90% CD11c+, ≥ 90% MHC class II+, ≥ 90% CD83+, ≥ 90% CD80+, and ≤ 5% CD14high by flow cytometry

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.