EasySep™ Direct HLA Crossmatch B Cell Isolation Kit
Immunomagnetic negative selection from whole blood, spleen, or lymph nodes kit
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EasySep™ Direct HLA Crossmatch B Cell Isolation Kit -
EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ -
Label for EasySep™ Direct HLA Crossmatch B Cell Isolation Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Dulbecco’s phosphate-buffered saline without calcium and magnesium -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.
Advantages:
• > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 99.8% purity of isolated cells
• Fast, easy-to-use, and column-free
• Isolated cells are untouched
• Up to 99.8% purity of isolated cells
• Fast, easy-to-use, and column-free
• Isolated cells are untouched
Components:
- EasySep™ Direct HLA Crossmatch B Cell Isolation Kit (Catalog #19684)
- EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
- EasySep™ Direct HLA Crossmatch B Cell Isolation Kit for RoboSep™ (Catalog #19684RF)
- EasySep™ Direct HLA Crossmatch B Cell Isolation Cocktail, 2 x 2.5mL
- EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
B Cells
Species:
Human
Sample Source:
Lymph Node; Spleen; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
HLA; Immunology
Related Products
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RoboSep™-S - The Fully Automated Cell Separator -
EasySep™ Human B Cell Isolation Kit
9-Minute cell isolation kit using immunomagnetic negative selection -
RosetteSep™ HLA B Cell Enrichment Cocktail
Immunodensity negative selection cocktail
Labeling Antibodies
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Anti-Human CD19 Antibody, Clone HIB19
Mouse monoclonal IgG1 antibody against human, chimpanzee CD19 -
Anti-Human CD20 Antibody, Clone 2H7
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD20 -
Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
19684
18F92330 or higher
19684RF
18F92330 or higher
19684RF
All
Educational Materials
(10)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with human whole blood from normal healthy donors, the B cell (CD19+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.8% (gated on CD45). In the above example, the B cell (CD19+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 12.3% and 98.4% (gated on CD45), respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.