Liver Epithelial Cell Research

Protocols for the propagation of liver organoids rely on culture conditions for the establishment and expansion of hepatic organoids, followed by a second culture environment that removes proliferative signals, inhibits ductal lineage commitment, and induces differentiation towards hepatocyte-like cells. During expansion, hepatic organoids express a ductal phenotype characterized by the expression of Krt19. However, upon differentiation, hepatic organoids downregulate ductal markers and exhibit albumin secretion, CYP3A activity, LDL uptake, and glycogen storage. Use our HepatiCult™ Organoid Kit (Human) to generate mature and functional human liver organoids that are suitable for further experimentation or biobanking.