Protocol Resources for Hybridoma Development

Protocols

Hybridoma Cell Development

Semi-solid cloning of hybridomas involves growing discrete, monoclonal colonies from individual cells immobilized within a viscous, semi-solid medium. Compared to conventional limting dilution cloning, semi-solid cloning significantly reduces the time and resources needed to produce monoclonal hybridomas. Explore these protocols and videos to learn how to perform semi-solid cloning to generate hybridomas efficiently, and with a high probability of monoclonality.

Protocol Integrating Electrofusion with the ClonaCell™-HY Workflow for Hybridoma Production

Tech Tip Best Practices for Screening Hybridoma Clones

Tech Tip Considerations for Immunizing Mice for Hybridoma Generation

Video Mammalian Cell Cloning Procedure: Customizable Semi-Solid Cloning with ClonaCell™ FLEX Medium

Video Customizable Semi-Solid Cloning of Mammalian Cells in 96-Well Plates Using ClonaCell™ FLEX Medium

Video ClonaCell™-HY Procedure

Technical Bulletin Protocol for Semi-Solid Hybridoma Cloning Using ClonaCell™-HY Medium in a 96-Well Plate

Tech Tip Optimizing Plating Density in Semi-Solid Media for Hybridoma Selection

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For detailed protocols developed specifically for our products, please consult the Product Information Sheet or enter the product keyword or catalog number below.

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