Protocol Resources for Hybridoma Development
Protocols
Hybridoma Cell Development
Semi-solid cloning of hybridomas involves growing discrete, monoclonal colonies from individual cells immobilized within a viscous, semi-solid medium. Compared to conventional limting dilution cloning, semi-solid cloning significantly reduces the time and resources needed to produce monoclonal hybridomas. Explore these protocols and videos to learn how to perform semi-solid cloning to generate hybridomas efficiently, and with a high probability of monoclonality.