ISSCR 2014 Innovation Showcase - Scalable Enzyme-Free Protocols for the Isolation and Maintenance of Human Induced Pluripotent Stem Cells (hiPSCs) Without Mechanical Colony Scraping
Dr. Erik Hadley, Senior Scientist at STEMCELL Technologies, gives a tutorial focusing on fibroblast reprogramming and hiPSC maintenance protocols that use ReLeSR™, an enzyme-free passaging reagent that eliminates manual removal of differentiated cells, colony scraping and complicated techniques to obtain uniform cell aggregates. When used with mTeSR™1 or TeSR™-E8™, ReLeSR™ enables the use of closed vessels, thus facilitating scale-up and automation. Applicability of ReLeSR™ in a complete workflow from fibroblast reprogramming to the establishment of a new hiPSC line are discussed. This video was filmed from the Innovation Showcase at ISSCR 2014 in Vancouver, BC.
Publish Date: December 22, 2014