What are Intestinal Organoids?
Intestinal organoids are three-dimensional (3D) in vitro tissue cultures that model the in vivo intestine. These organoids include a polarized
epithelial layer that surrounds a functional lumen and contains cell types of the intestinal epithelium present in proportions and relative spatial
arrangement that mimic what is observed in vivo. As the technology has matured, intestinal organoids have found application in a wide variety of
research fields, including upstream drug discovery and patient-specific drug screening, cancer and immunology studies, and the pathogenesis of
In the last decade there has been a dramatic shift in the availability of tools and model systems used to study the intestinal epithelium.
Since the introduction of the mouse small intestinal organoid model in 2009,1 there has been an avalanche of developments in this
field, including development of culture conditions for human organoids derived from primary colonic tissue,2 as well as from human
pluripotent stem cells (hPSCs).3 Various experimental techniques have also been developed in parallel with, and applied to, intestinal
organoid cultures with a scientifically synergistic effect. Some of these techniques include novel tools for genetic manipulation,4,5
approaches for in vitro disease modelling6–9 and innovative co-culture system with autologous cell types10,11 or
bacteria,12–14 as well as viral infection models.15,16 The development of such techniques as applied to intestinal organoid
cultures has vastly increased the utility of this model system for a wide variety of purposes.
The Intestinal Epithelium
The intestinal epithelium incorporates several distinct cell populations, including the rapidly dividing intestinal stem cells (ISCs) that
facilitate the typical four-to-five day turnover cycle of the adult intestinal epithelium.17 This property of rapid regeneration at
intestinal stasis makes the intestine a uniquely convenient model system for epithelial cell biology and adult stem cell biology studies both
inside and outside the specific context of intestinal function. The intestinal niche has been well-characterized, and shown to consist of spatial
gradients of high WNT and Epithelial Growth Factor (EGF), while Bone Morphogenetic Protein (BMP) signals are inhibited. Understanding of these niche
signals heavily informed development of the culture conditions for intestinal organoids.
Figure 1. Diagram of the small intestinal epithelium highlighting the identity and spatial arrangement of key epithelial cell types.
The adult intestinal epithelium is primarily composed of six cell types that are arranged in a crypt-villus structure (Figure 1).18 At the base of the intestinal crypt, the ISCs are found intercalated with Paneth cells,17 credited with much of the signaling required to maintain the stem cell niche. Transit amplifying cells are partially differentiated cells that migrate upward via a physical crypt exclusion mechanism as the ISCs below them divide. As these cells move upward out of the crypt, they move along signaling gradients that trigger them to differentiate, giving rise to the mature cell types that populate the villus domain. Mature cells include enterocytes, which make up the majority of the villus epithelium and carry out nutrient absorption; goblet cells, which secrete mucus to protect the epithelial lining and help move intestinal contents through the lumen; and enteroendocrine cells, which respond to chemical actuators in the luminal contents by secreting hormones into the body to maintain nutrient metabolism.
Wallchart: The Intestinal Crypt
Decorate your bench space with this handy overview of the intestinal stem cell niche. Request a free copy of the Cell SnapShot: The Intestinal Crypt for your lab.
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Types of Intestinal Organoids
Organoids Derived from Primary Intestinal Tissue
The seminal work on the intestinal organoid culture system that came out of the laboratory of Hans Clevers in 20091 describes a culture
system wherein the stem cell niche of the adult intestinal stem cells is recapitulated in vitro. This allows for establishment of organotypic
intestinal epithelial cell cultures that maintain the regenerative properties of the in vivo intestine. The first protocol published by Sato et
al.1 described the isolation of intestinal crypts from mouse intestinal tissue and their subsequent culture to form organoids. Intestinal
crypts are embedded in a dome of Matrigel® extracellular matrix and submerged in a growth medium that contains specific growth factors designed
to mimic the signaling present at the intestinal crypt base in vivo.
When cultured using this method, the organoids form an epithelial monolayer surrounding a central lumen. Corresponding to the in vivo intestinal
epithelium, the cells that make up the organoid epithelium are extruded and sloughed off into the lumen of the organoid. This results in the
collection of cellular debris within the lumenal compartment over time and the associated decline in viability of the cultures, even in the
presence of appropriate growth factors. The organoid cultures are therefore passaged periodically by dissociating the organoids from the
Matrigel® and breaking them into fragments which are reseeded in new cultures. This process can be repeated indefinitely with remarkable
genetic stability,19 and constitutes an effective expansion method for the intestinal stem cell population.
There have been many advancements and modifications to the basic culture methodology first developed for mouse and human intestinal epithelial
organoids. These subsequent studies have introduced methodologies for specifically adapting the culture system for patient tumor-derived tissues
with different growth factor dependencies21,22; growth of organoids in formats appropriate for medium- to high-throughput compound
screening23,24; and use of defined extracellular matrix systems,25 among other application-specific adaptations.
Organoids Derived from Pluripotent Stem Cells
Intestinal organoids are established from undifferentiated cells through the differentiation of hPSCs to definitive endoderm and initiation of
mid/hindgut specification, then through intestinal fate induction in 3D culture as intestinal organoids.3 Interestingly, the
formation of 3D cultures occurs spontaneously in these cultures in the course of directed differentiation to hindgut; spheroids exhibiting hindgut
markers bud out of the monolayer cultures and can be easily isolated from the culture supernatants. Similar to primary tissue-derived organoids,
the partially differentiated spheroids are then embedded in Matrigel® domes and incubated in cell culture medium that promotes their
differentiation to the intestinal lineage and subsequent partial maturation.3
In contrast to the intestinal epithelial organoids grown from isolated crypts or LGR5+ intestinal stem cells, intestinal organoids derived from
differentiated hPSCs incorporate a mesenchymal compartment that contributes to the intestinal stem cell niche signaling present in the
cultures.18 Intestinal organoids derived from directed differentiation of hPSCs also exhibit characteristics reminiscent of the
longer-term processes that take place during normal tissue development. At early passages the organoids are characterized by a distinctly fetal
intestinal phenotype whereas full maturation of the intestinal epithelial cells is thus far only achievable through transplantation of the organoids
into mouse kidney capsules.26 Recapitulation of intestinal development in the hPSC-derived organoid system makes this model an excellent
in vitro tool for examining intestinal developmental milestones in an easy-to-manipulate experimental system.18
Experimental Techniques Applied to Organoid Cultures
The widespread uptake and application of intestinal organoid cultures across a variety of fields is in great part due to the multitude of
experimental tools and approaches to which these cultures are amenable. Since the introduction of the basic organoid culture methodology,
there have been many advances both developing and validating various techniques in the intestinal organoid model system.
A key functionality of the intestinal organoid system is the ability to genetically manipulate the cells that comprise the culture,
especially in comparison to the challenges of performing similar manipulations in vivo. Intestinal organoid cultures can be manipulated
genetically through a variety of means, including the introduction of genetic material via retro-, adeno- and lentiviral
infection27–29 or electroporation,30 as well as specific site-directed mutagenesis using CRISPR/Cas9
gene editing.31 Once subjected to a specific technique for genetic modification, organoid cultures can be reestablished
clonally from single-cell suspensions of sorted stem cells1,2 or whole dissociated organoids, enabling isolation of the desired
genetic variant for further propagation. For the mouse intestinal model, this presents a significant advantage over the time and energy
required to create a specific knock-out mouse strain. For the human intestinal model, the ability to manipulate the genetic makeup of
the cells in vitro presents the opportunity to exercise a level of control over the genetic composition of the model that would otherwise
be impossible using traditional methods of primary cell culture or immortalized cell lines.
In addition to their compatibility with direct genetic manipulation in vitro, organoid cultures are also a highly informative way to
examine pre-existing knockout models to probe the mechanisms of specific cellular processes or disease pathologies.
Genome Editing of Human Intestinal Organoids
Explore this validated protocol for high-efficiency CRISPR-Cas9 gene editing of adult stem cell-derived intestinal organoids.
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Intestinal organoid cultures are highly passageable while maintaining a remarkable level of genetic and phenotypic stability.32
However, it is sometimes advantageous to create a static library of organoid cultures through cryopreservation of the cultures. This is
especially true in the context of creating cryopreserved libraries of patient-derived organoids that maintain the properties of the original
culture and can be used in applications like preclinical drug efficacy screening.23
The isolation of the epithelium from other bodily systems as organoids presents an advantage to experimental specificity, but also a
limitation with respect to investigating specific interactions with other autologous or foreign cells. This issue is being addressed by
the constant development of new co-culture systems for intestinal organoids with various other cell types. Intestinal organoids have been
cultured with immune cells10,11,33 by introduction of the non-epithelial cells in the extracellular matrix supporting the organoid
culture, thus recapitulating the interactions expected to take place at the basolateral side of the epithelium. A functional enteric nervous
system has also been generated through the co-culture of hPSC-derived organoids and neural crest cells, establishing a model that allows the
study of human gastrointestinal motility disorders.34 Similarly, live bacteria have been introduced into the lumen of organoid
cultures via microinjection to model interactions that occur at the apical side of the epithelium in vivo.12–14 Organoids have also
been validated as model systems for viral infection.15,16
While intestinal organoids can increase flexibility and precision in experimental design, intestinal organoids are limited by the presence
of a closed luminal compartment, a physical characteristic that presents challenges for specific experiments. More recently, protocols have
described how to grow organoids in 2D as organoid-derived monolayers. Growing intestinal organoids as a monolayer enables easy access to the
apical side of the epithelium, facilitating studies involving apical cell surface receptors, interaction with commensal or pathogenic
microorganisms, or modeling the effect of potentially beneficial or harmful compounds in intestinal contents. Intestinal epithelial monolayer
cultures can be established on glass coverslips that enable high-quality imaging, or on Transwell® permeable supports that enable
measurement of active and passive transport of substances across the epithelial layer, as well as electrical barrier function assays.
Efficient Establishment and Growth of Human Intestinal Organoid-Derived Monolayers
Explore the poster presented at ISSCR 2019.
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Applications of Intestinal Organoids
While the roots of the organoid culture system lie within the developmental biology community, intestinal organoid cultures are now feeding
back valuable information about the developmental trajectory of the tissue they were designed to model.35 This is especially true
for hPSC-derived intestinal organoids, which follow the overall intestinal developmental pathways during their establishment3 and
maintain a more fetal-like phenotype in organoid culture.36 The great utility of organoid cultures in this context lies in part in
the facile accessibility to a continuum of developmental timepoints, a stark contrast to what is otherwise available with respect to human
Perhaps the most obvious application of intestinal organoid cultures is as an in vitro model system for the intestinal epithelium. As these
cultures incorporate all of the epithelial cell types present in the tissue and recapitulate many of the inter- and intra-epithelial cell
dynamics that occur in vivo, intestinal organoid cultures constitute a valuable experimental tool for probing intestinal epithelial cell
biology. As such, this in vitro model system has recently been used to investigate intestinal regeneration,37,38 the intestinal
stem cell niche,39–44 intestinal inflammation,9,45–48 intestinal stem cell mutation rates,49 and the function of
terminally differentiated intestinal epithelial cells (eg. nutrient sensing and hormone secretion).50–52
Intestinal organoids also have applications outside of tissue-specific biological processes and conditions. This is due to the active stem
cell population and resultant high turnover of the intestinal epithelium compared to many other tissues. The active nature of the intestinal
stem cell niche lends it great utility as a model system to investigate more general questions regarding adult stem cell biology or epithelial
cell biology. Specific examples of applications that this technology has recently been applied to include investigating the shared mechanisms
between adult stem cell proliferation and cancer,53 mechanisms of protein degradation,54 and epithelial
Intestinal organoid cultures are also being used to model intestinal diseases as well as epithelial diseases in other tissues. For instance,
organoids grown from patients carrying mutations in the myosin 5b gene can be used as a model for microvillus inclusion disease (MVID) which
results in improper localization of proteins on the apical surface of the intestinal epithelium and enterocyte polarization.8 This
model allowed for organoids to be generated from patients exhibiting MVID symptoms but not carrying mutations in the myosin 5b gene. When
sequenced, these samples identified the syntaxin 3 gene as a contributor to the loss of microvilli.
Cultures can be derived from adjacent healthy and diseased (tumorigenic) tissue obtained from the same patient,23 an approach
that enables evaluation of the disease state while controlling for potentially confounding factors in the healthy specific genetic background.
The analysis of the genetic composition of these unique tumors allow for correlations to be made between organoid phenotype and specific
mutation combinations. Multiple studies have demonstrated both the detection of mutations in the combination of APC, KRAS, SMAD4 and
TP53,21 as well as used gene editing through the CRISPR/Cas9 system to model the adenoma-carcinoma sequence.31
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Drs. Sylvia Boj, Robert Vries, and Ryan Conder discuss the use of organoids as model systems for studying human health and disease.
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The colon can also be exploited for modeling diseases not specific to the intestine such as Cystic Fibrosis, which usually manifests in the
lung as a result of mutations in the cystic fibrosis transmembrane receptor (CFTR). Biopsies from patients carrying mutations in the CFTR gene
do not respond to an organoid swelling assay, allowing for the positive identification of such mutations.56
Drug Development and Screening
One of the most promising aspects of research using organoids as a model is the possibility to screen cultures derived from individual
patient tissue samples for effects of external treatments. With the quick culture times and ability to scale up samples, organoid culture
has already been used in specific patient screening. For example, the previously mentioned CFTR swelling assay, designed by Beekman and
colleagues,56 which provides a readout for response to current drugs or combinations of drugs which improve the transport of
chloride through ion channels in patients with Cystic Fibrosis. In the first trial of the CFTR swelling assay, a drug combination able to
clear much of the mucus that had accumulated in the patient's lung was identified, therefore providing a proof of principle experimental
design that is currently moving towards being adopted into government screening platforms.
A key aspect that is still being optimized in organoid culture conditions is the transition to high throughput screening. Steps toward
this were recently demonstrated by the development of an automated platform in 384-well format for organoid cultures established from tissue
samples of colon cancer patients.24 These organoids were subjected to various drug compounds, and the assay validated for robustness
and reproducibility, therefore providing a system that provides more physiological relevance than 2D cultures without sacrificing screening
A reciprocal approach addresses more specific treatments for the mutation composition of patient sample libraries using a specific drug.
The van Lohuizen group treated organoid cultures established using tissue samples taken from 18 patients diagnosed with colorectal cancer
with an EZH2 inhibitor, GSK126.28 Although a variety of responses were observed, associations with ATRX and PAX2, as well as a
correlation with BIK expression and Nutlin-3A were found. These findings demonstrate the feasibility of upstream pharmaceutical development
based on patient organoid library screening.
Functional Applications Using Organoids
Dr. Ryan Conder describes some of the latest applications of organoid model systems.
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Outlook for Intestinal Organoids
Since the discovery that intestinal stem cells can establish organoid structures recapitulating the mammalian intestine, the organoid model
has been quickly adopted by the research community. This rapid acceptance of organoids as a research tool has been facilitated by two key
aspects, the physiological relevance to the intestine and the improvements over traditional two-dimensional culture systems. For
translational-based research, organoids provide the possibility of high throughput analysis of samples from individual patients bridging
the gap between basic research and precision medicine. The ability to generate samples for marker or genome-based analyses, as well as drug
or small molecule treatment, are possible in the same experimental design.
These cultures also provide the promise of expanding the understanding of basic stem cell biology which will be a necessary complement to the translational
studies. By the very nature of the culturing system, early stem cell divisions and tissue formation will be accessible to experimental assay and
manipulation via CRISPR/Cas9 gene editing.
As more organoid-based systems are established, a combining of several specific cell types into the same organoids becomes feasible. This co-culturing
will allow for the identification of the specific roles of each component stem cell niche as well as the co-operative and necessary roles of the individual
The applications of the organoid culture system continue to expand, and as they do so will our understanding of the complex interactions of
the organs that comprise the human body, the mechanisms that have malfunctioned in specific diseases, and the ability to rapidly treat patients
of those diseases.
Read key insights and watch the webinars from the Nature Reseach Roundtable on Organoids.
Growing Organoids Wallchart
Decorate your lab with a handy overview of epithelial organoid systems.
Organoid Resource Center
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