Cell-permeant, cationic fluorescent dye used to measure mitochondrial membrane potential
• Excitation wavelength: 552 nm
• Emission wavelength: 574 nm
• Retention dependent on mitochondrial inner membrane potential
• Verified for use with flow cytometry
TMRE is a cell-permeant, cationic compound that emits a fluorescent red-orange signal. It readily accumulates in the mitochondria with high membrane potential, whereas depolarized mitochondria fail to retain the dye.
TMRE has a sufficient retention time that is optimal for cell sorting for further purification and characterization (Barteneva et al. J Histochem Cytochem, 2014). TMRE does not inhibit the mitochondrial function, and can be used in the identification of apoptotic cells (Barteneva et al., 2014).
Figure 1. TMRE (Perchlorate) Is Not Retained in Apoptotic Cells
Flow cytometry analysis of Jurkat cells labeled with TMRE after incubation in the presence or absence of staurosporine, an inducer of apoptosis.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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