• Optimized for use with StemSpan™ media. When combined with StemSpan™ SFEM II in particular, supports up to 4-fold higher expansion of erythroid cells from human CD34+ CB cells than other serum-free media on the market.
• Supplied as a 100X concentrate. After thawing and mixing, the tube contents can be added directly to any hematopoietic cell expansion medium of choice.
• Recombinant human interleukin 3 (IL-3)
• Recombinant human erythropoietin (EPO)
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. Production of Erythroblasts by Expansion and Lineage Specific Differentiation of CD34+ Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing Erythroid Expansion Supplement
Flow cytometry dot plots showing expression of the HSPC marker CD34 and erythroid markers CD71 and glycophorin-A (GlyA) (A) before culture and (B,C) after 14 days of culture of enriched CD34+ CB cells in StemSpan™ SFEM containing Erythroid Expansion Supplement. The frequency of CD34+ cells declined from ~60% before culture to <0.1% after 14 days. In parallel, erythroid cells gradually accumulated from levels of <1% before culture to >90% by day 14. The bulk of cell population recovered from 14-day culture consisted of CD71+GlyA+ erythroblasts. More immature CD71+GlyA- progenitors and proerythroblasts, as well as more differentiated CD71-/low GlyA+ normoblasts were also present at low frequencies.
Table 1. Production of Erythroid Cells From CD34+ Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing Erythroid Expansion Supplement
Numbers and percent of erythroid cells produced after 14 days of culture of enriched CD34+ cells from 14 different CB samples. *95% confidence limits; the range within which 95% of the results will typically fall.
Figure 2. Comparison of Erythroid Cell Expansion in Different StemSpan™ Media Containing Erythroid Expansion Supplement
(A) Average numbers and (B) frequencies of erythroid cells normalized relative to the values obtained in StemSpan™ SFEM (grey bars) after culturing purified CD34+ CB cells (n=6) for 14 days in StemSpan™ SFEM, SFEM II (gold bars) or ACF (orange bars) media containing Erythroid Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results typically fall. Erythroid cell yields were significantly higher in SFEM II compared to ACF (p<0.01). Erythroid cell yields in SFEM II were also significantly higher than in SFEM for 5 of 6 CB samples tested (p<0.05).
Table 2. StemSpan™ Serum-Free Expansion Media and Erythroid Expansion Supplement