ACCUMAX™

Cell detachment solution

ACCUMAX™

Cell detachment solution

From: 178 USD
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Cell detachment solution
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Product Advantages


  • Increase the accuracy of cell counts, using manual or automated methods

  • Effectively dissolve cell clumps, such as spheroids and neurospheres

  • Obtain cleaner cultures with a ready-to-use solution, free from mammalian and bacterial-derived products


Overview

Generate single-cell suspensions from clumped cell cultures for accurate cell counting with ACCUMAX™. This ready-to-use solution of proteolytic and collagenolytic enzymes effectively dissolves cell clumps and effectively facilitates the detachment of cells from primary tissue, as well as standard and adhesion-coated culture plasticware.

ACCUMAX™ is free of mammalian- or bacterial- derived products to ensure reproducible and reliable experiments, and has been shown to work effectively across a wide variety of cell types. Each lot of ACCUMAX™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromogenic tetrapeptides), and cell detachment from tissue culture plastic.
Contains
• 1X ACCUMAX™ enzymes in Dulbecco’s phosphate-buffered saline (D-PBS)
• 0.5 mM EDTA•4Na
Subtype
Enzymatic
Cell Type
Pluripotent Stem Cells
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Culture
Area of Interest
Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
ACCUMAX™
Catalog #
07921
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ACCUMAX™
Catalog #
07921
Lot #
All
Language
English

Resources and Publications

Publications (1)

Transcriptome Dynamics of Developing Photoreceptors in Three-Dimensional Retina Cultures Recapitulates Temporal Sequence of Human Cone and Rod Differentiation Revealing Cell Surface Markers and Gene Networks. Kaewkhaw R et al. Stem cells (Dayton, Ohio) 2015 DEC

Abstract

The derivation of three-dimensional (3D) stratified neural retina from pluripotent stem cells has permitted investigations of human photoreceptors. We have generated a H9 human embryonic stem cell subclone that carries a green fluorescent protein (GFP) reporter under the control of the promoter of cone-rod homeobox (CRX), an established marker of postmitotic photoreceptor precursors. The CRXp-GFP reporter replicates endogenous CRX expression in vitro when the H9 subclone is induced to form self-organizing 3D retina-like tissue. At day 37, CRX+ photoreceptors appear in the basal or middle part of neural retina and migrate to apical side by day 67. Temporal and spatial patterns of retinal cell type markers recapitulate the predicted sequence of development. Cone gene expression is concomitant with CRX, whereas rod differentiation factor neural retina leucine zipper protein (NRL) is first observed at day 67. At day 90, robust expression of NRL and its target nuclear receptor NR2E3 is evident in many CRX+ cells, while minimal S-opsin and no rhodopsin or L/M-opsin is present. The transcriptome profile, by RNA-seq, of developing human photoreceptors is remarkably concordant with mRNA and immunohistochemistry data available for human fetal retina although many targets of CRX, including phototransduction genes, exhibit a significant delay in expression. We report on temporal changes in gene signatures, including expression of cell surface markers and transcription factors; these expression changes should assist in isolation of photoreceptors at distinct stages of differentiation and in delineating coexpression networks. Our studies establish the first global expression database of developing human photoreceptors, providing a reference map for functional studies in retinal cultures.