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R-Spondin-3 is a member of thrombospondin type 1 repeat (TSR-1) superfamily that is involved in the canonical Wnt/β-catenin signaling pathway (de Lau et al.). R-spondin proteins are characterized by two furin-like repeats at the amino terminus and thrombospondin domain located near the carboxyl terminus (de Lau et al.). R-spondin-3 expression is associated with ovarian cancer (Gu et al.), prostate cancer (Mesci et al.), and differentiation of intestinal epithelial cells in diabetes mellitus (Shan et al.). In a transgenic mice model, the expression of R-Spondin-3 induces the expansion of Lgr5+ stem cells, Paneth cells, and Lgr4+ cells, promoting the intestinal stem cell compartment (Hilkens et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain.
(A) The binding activity of Human Recombinant R-Spondin-3 was tested by functional ELISA with immobilized Human Recombinant R-Spondin-3 at 2000 ng/mL. Immobilized Human Recombinant R-Spondin-3 can bind human RNF43 hFc with a linear range of 3 - 12 ng/mL.
(B) Human Recombinant R-Spondin-3 was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant R-Spondin-3 has a predicted molecular mass of 15.3 kDa, but the apparent molecular mass is approximately 22 kDa to 27 kDa due to different glycosylation.
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