EasySep™ Mouse Biotin Positive Selection Kit II

Immunomagnetic positive selection kit
Catalog #
17665_C
Immunomagnetic positive selection kit
From: 455 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Mouse Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556).

For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655).
Advantages
• Fast and easy-to-use
• No columns required
Components
  • EasySep™ Mouse Biotin Positive Selection Kit II (Catalog #17665)
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
  • RoboSep™ Mouse Biotin Positive Selection Kit II (Catalog #17665RF)
    • EasySep™ Biotin Selection Cocktail, 1 mL
    • Mouse FcR Blocker, 0.1 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English

Educational Materials (7)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Technical Bulletin
Endothelial Protein C Receptor (EPCR): A New Marker for Identification and Positive Selection of Mouse Hematopoietic Stem Cells
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Data and Publications

Data

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 97.5%, respectively, using a biotinylated anti-mouse CD4 antibody and EasySep™ Mouse Biotin Positive Selection Kit II (as assessed using PE-conjugated streptavidin).

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