EasySep™ Mouse Biotin Positive Selection Kit II
Immunomagnetic positive selection kit
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EasySep™ Mouse Biotin Positive Selection Kit II -
EasySep™ Mouse Biotin Positive Selection Kit II -
Label for EasySep™ Mouse Biotin Positive Selection Kit II
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer
Overview
The EasySep™ Mouse Biotin Positive Selection Kit II is designed to isolate cells that are labeled with biotinylated antibodies. Desired cells are targeted with antibody complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556).
For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655).
This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556).
For highly purified particle-free cells, we recommend the EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655).
Advantages:
• Fast and easy-to-use
• No columns required
• No columns required
Components:
- EasySep™ Mouse Biotin Positive Selection Kit II (Catalog #17665)
- EasySep™ Biotin Selection Cocktail, 1 mL
- Mouse FcR Blocker, 0.1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Mouse Biotin Positive Selection Kit II (Catalog #17665RF)
- EasySep™ Biotin Selection Cocktail, 1 mL
- Mouse FcR Blocker, 0.1 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- RoboSep™ Vial For Primary Conjugated Antibody (not required for manual use), 1 vial
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Mesenchymal Stem and Progenitor Cells; Monocytes; Mononuclear Cells; Myeloid Cells; NK Cells; Other; Plasma Cells; T Cells; B Cells; Macrophages; Dendritic Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells; Marrow Stromal Cells
Species:
Mouse
Sample Source:
Bone Marrow; Other; Spleen
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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EasySep™ Mouse PE Positive Selection Kit II
Immunomagnetic positive selection kit -
EasySep™ Mouse APC Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit -
EasySep™ Mouse FITC Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit
Scientific Resources
Product Documentation
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Educational Materials
(7)
Data and Publications
Data
Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 97.5%, respectively, using a biotinylated anti-mouse CD4 antibody and EasySep™ Mouse Biotin Positive Selection Kit II (as assessed using PE-conjugated streptavidin).
Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.