EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

From: 826 USD
Catalog #
(Select a product)
Immunomagnetic positive selection cell isolation kit
Add to Wish List

Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySepSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit (Catalog #17884)
    • EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit with Filter Tips (Catalog #17884RF)
    • EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol

Overview

Isolate highly purified CD33+ and CD66b+ myeloid cells, including myeloid progenitor cells, granulocytes, and monocytes, from fresh whole blood prior to chimerism testing.

Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off.

This product has replaced the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) for faster cell isolations. For even higher efficiency, this product can be used with RoboSep™ for automated immunomagnetic cell isolation.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Centrifugation Tubes
Cell Type
Myeloid Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Positive
Brand
EasySep
Area of Interest
Chimerism, Immunology

Data Figures

Starting with human whole blood, the myeloid cell content of the isolated fraction is typically 94.5 ± 4.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 66.6% and 98.5%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17884RF
Lot #
All
Language
English
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17884RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17884
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17884
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more