Rhod-2 AM (Bromide)
A cell-permeable calcium dye that exhibits fluorescence upon increasing Ca2+ concentrations
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• Excitation wavelength: 553 nm
• Emission wavelength: 577 nm
• Dissociation constant (Kd) = 570 nM
• Verified for use in fluorescence microscopy
Rhod-2 AM is a cell-permeable acetoxymethyl (AM) ester dye, which fluoresces in the presence of Ca2+. Cellular uptake of Rhod-2 AM is facilitated by the AM group, which is removed by cytoplasmic esterases, resulting in intracellular accumulation (Smith et al. Sci Signal, 2018). Rhod-2 AM selectively accumulates in the mitochondria. Increasing Ca2+ concentrations significantly enhance Rhod-2 AM fluorescence. The long emission wavelengths of this dye make it valuable for applications involving cells that have high levels of autofluorescence.
Increased intracellular calcium concentrations are a critical indicator of activation of apoptotic pathways. (Mukherjee et al. J Biol Chem, 2002). Use Rhod-2 AM (Bromide) dye to measure mitochondrial calcium influx by detection using flow cytometry, fluorescence microscopy, and microplate readers.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Rhod-2 AM (Bromide)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.