Mitochondrial Tracking Dye, Blue
Fluorescent hydrophobic dye that selectively accumulates in mitochondria
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Overview
Features:
• Excitation wavelength: 344 nm
• Emission wavelength: 469 nm
• Easily crosses live cell membranes due to hydrophobicity
• Partially dependent on mitochondrial membrane potential
• Cell-retaining group produces longer retention times
Mitochondrial Tracking Dye, Blue is a hydrophobic dye that accumulates in mitochondria and is partially dependent on mitochondrial membrane potential. Upon depolarization, the dye relocalizes to the cytoplasm and becomes non-specific, but still retains its blue fluorescence. This mitochondrial tracking dye contains a cell-retaining group—thiol chloromethyl—that increases staining efficiency by retaining fluorescence long after fixation (Clutton et al. Cytometry A, 2019). Mitochondrial Tracking Dye, Blue is suitable for multi-labeling experiments such as microplate assays and flow cytometry. Use mitochondrial tracking dyes to quantify mitochondrial activity, localization, and abundance.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.