Caspase-3/7 Activity Plate Reader Assay Kit, Blue

For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis

Caspase-3/7 Activity Plate Reader Assay Kit, Blue

For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis

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For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis
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Product Advantages


  • Ac-DEVD-AMC detects caspase-3/7 activity

  • Excitation wavelength: 341 nm

  • Emission wavelength: 441 nm

  • Easily scalable for high-throughput assays

  • Verified for use with fluorescent plate readers

What's Included

  • Caspase-3/7 Activity Plate Reader Assay Kit, Blue (Catalog #100-0921)
    • Caspase-3/7 Substrate (200X), Blue, 2 x 50 µL
    • Assay Buffer, 20 mL

Overview

Efficiently detect caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Plate Reader Assay Kit, Blue, which includes the fluorogenic substrate Ac-DEVD-AMC and an assay buffer. This reagent kit uses a quick and simple staining protocol that is well suited for high-throughput screening and compatible with standard fluorescent plate readers.

Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). When added to apoptotic cells, Ac-DEVD-AMC is cleaved by activated caspase-3/7, generating a blue fluorescent signal that can be detected by standard plate readers.

Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases—caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase assays are useful for quantifying caspase-3/7 activity in apoptotic cells and for screening caspase-3/7 inhibitors.
Area of Interest
Cancer
CAS Number
67-68-5

Data Figures

Figure 1. Caspase-3/7 Activity Plate Reader Assay Kit, Blue

Jurkat cells were treated with 0.2% DMSO (Control) or 1 µM staurosporine (STSP), and in the presence or absence of Z-VAD-FMK (a pan-caspase inhibitor). Caspase-3/7 activity was assessed as a measure of fluorescence using the Caspase-3/7 Activity Plate Reader Assay Kit, Blue. Data are shown as the mean from two independent experiments.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Catalog #
100-0921
Lot #
All
Language
English
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Safety Data Sheet 1
Catalog #
100-0921
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All
Language
English
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Safety Data Sheet 2
Catalog #
100-0921
Lot #
All
Language
English