ImmunoCult™-ACF Human B Cell Expansion Supplement
Serum-free and animal component-free culture supplement for expansion of human B cells
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ImmunoCult™-ACF Human B Cell Expansion Supplement -
Label for ImmunoCult™-ACF Human B Cell Expansion Supplement
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Required Products
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ImmunoCult™-XF T Cell Expansion Medium
Serum-free and xeno-free medium for the expansion of human T cells
Overview
ImmunoCult™-ACF Human B Cell Expansion Supplement is a serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.
ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) to prepare a complete serum-free medium for culturing and expanding human B cells.
ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) to prepare a complete serum-free medium for culturing and expanding human B cells.
Advantages:
⦁ Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
⦁ Serum-free and animal component-free supplement
⦁ Serum-free and animal component-free supplement
Contains:
Recombinant proteins, including CD40L and other cytokines
Chemically defined components
Subtype:
Supplements
Cell Type:
B Cells
Species:
Human
Application:
Activation; Cell Culture; Differentiation; Expansion; Genome Editing
Brand:
ImmunoCult
Area of Interest:
Antibody Development; Immunology; Infectious Diseases
Formulation:
Animal Component-Free; Serum-Free
Related Products
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EasySep™ Human Pan-B Cell Enrichment Kit
Immunomagnetic negative selection cell isolation kit -
EasySep™ Human CD19 Positive Selection Kit II
Immunomagnetic positive selection kit -
EasySep™ Human Memory B Cell Isolation Kit
Immunomagnetic cell isolation kit using particle release technology -
Human Peripheral Blood B Cells, Frozen
Primary human cells, frozen
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Data
Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™-ACF Human B Cell Expansion Supplement
B cells isolated from human peripheral blood mononuclear cells (PBMCs) (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1,190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.
Figure 2. Light Microscopy Image of Cultured Human B Cells
B cells isolated from human PBMCs (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged on day 4 after seeding and imaged at 40x magnification on day 6.
Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement
Memory B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each time point. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Figure 4. Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement
Naive B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each time point. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Figure 5. Expansion of Human Pan-B Cells Cultured with Various Base Media Supplemented with ImmunoCult™-ACF Human B Cell Expansion Supplement or 10% FBS
Pan-B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Pan-B Cell Isolation Kit and seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with various base media supplemented with either ImmunoCult™-ACF Human B Cell Expansion Supplement or 10% FBS. The cells were passaged every 3 - 4 days and the fold expansion of viable cells was calculated at each time point. Data represent the mean + 1SD for n = 3 donors (each culture condition was performed in triplicate).
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.