Anti-Human CD25 Antibody, Clone BC96

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25
Catalog #
60158_C
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25
From: 53 USD

Overview

The BC96 antibody reacts with human CD25, a 55 kDa type I integral transmembrane glycoprotein. CD25 is involved in the activation and proliferation of lymphocytes and can be used in the identification of T cell subsets in viral infections like HIV and HTLV. CD25 is present on activated T and B cells, monocytes, macrophages, and regulatory T cells. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for regulatory T cells. CD25 has a low affinity for its IL-2 ligand but associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2 receptor.
Subtype
Primary Antibodies
Target Antigen
CD25
Alternative Names
IL-2 Receptor alpha chain, IL-2Ra, IL2Ra, Tac, p55
Reactive Species
Baboon, Cynomolgus, Rhesus, Chimpanzee, Human, Macaque
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
B Cells, T Cells, T Cells, Regulatory
Application
Flow Cytometry
Area of Interest
Cancer, Cell Therapy, Immunology, Transplantation Research
Clone
BC96
Gene ID
3559
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96
Catalog #
60158
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, Alexa Fluor® 488
Catalog #
60158AD, 60158AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, APC
Catalog #
60158AZ, 60158AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, Biotin
Catalog #
60158BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, FITC
Catalog #
60158FI, 60158FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, PE
Catalog #
60158PE, 60158PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96
Catalog #
60158
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, Alexa Fluor® 488
Catalog #
60158AD, 60158AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, APC
Catalog #
60158AZ, 60158AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, Biotin
Catalog #
60158BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, FITC
Catalog #
60158FI, 60158FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD25 Antibody, Clone BC96, PE
Catalog #
60158PE, 60158PE.1
Lot #
All
Language
English

Educational Materials (2)

Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, Alexa Fluor® 488 (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD; solid line histogram).

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