Anti-Human CD25 Antibody, Clone BC96

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25
From: 47 USD

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD25
From: 47 USD

Overview

The BC96 antibody reacts with human CD25, a 55 kDa type I integral transmembrane glycoprotein. CD25 is involved in the activation and proliferation of lymphocytes and can be used in the identification of T cell subsets in viral infections like HIV and HTLV. CD25 is present on activated T and B cells, monocytes, macrophages, and regulatory T cells. Expression of CD25, together with CD4 and FOXP3, is considered a phenotypic signature for regulatory T cells. CD25 has a low affinity for its IL-2 ligand but associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2 receptor.
Subtype:
Primary Antibodies
Target Antigen:
CD25
Alternative Names:
IL-2 Receptor alpha chain, IL-2Ra, IL2Ra, Tac, p55
Reactive Species:
Human; Baboon; Chimpanzee; Cynomolgus; Rhesus; Macaque
Conjugation:
Unconjugated; APC; FITC; PE; Alexa Fluor 488; Biotin
Host Species:
Mouse
Cell Type:
T Cells, Regulatory; B Cells; T Cells
Application:
Flow Cytometry
Area of Interest:
Immunology; T Cell Engineering; Transplantation Research; Cancer Research
Clone:
BC96
Gene ID:
3559
Isotype:
IgG1, kappa

Scientific Resources

Educational Materials

(3)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60070), followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, PE (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60070PE; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, APC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60070AZ; solid line histogram).

Flow cytometry analysis of PHA-L-activated human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD25 Antibody, Clone BC96, Alexa Fluor® 488 (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (Catalog #60070AD; solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.