Nicotinamide

Cell culture supplement for differentiation of ES and iPS cells
Nicotinamide

Cell culture supplement for differentiation of ES and iPS cells

100 g
Catalog # 07154
111 USD

Overview

Nicotinamide (NAM) is a cell culture supplement used in the differentiation of embryonic stem (ES) and induced pluripotent stem (iPS) cells. It is an amide derivative of vitamin B3, a poly (ADP-ribose) polymerase (PARP) inhibitor, and represents the primary precursor of NAD+. Nicotinamide has also been shown to modulate stem cell differentiation in various applications, most notably for the differentiation of pancreatic mouse ES and iPS cells to pancreatic islet-like insulin-secreting cells.
Cell Type
Endoderm, PSC-Derived, Pancreatic Cells, Pluripotent Stem Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Differentiation, Expansion
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
CAS Number
98-92-0

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Nicotinamide
Catalog #
07154
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Nicotinamide
Catalog #
07154
Lot #
All
Language
English

Educational Materials(2)

Brochure
Small Molecules for Stem Cell Research
Wallchart
Directed Differentiation of Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications (1)

The Journal of clinical investigation 1993 SEP Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. Otonkoski T et al.

Abstract

The effects of nicotinamide (NIC) on human fetal and adult endocrine pancreatic cells were studied in tissue culture. Treatment of the fetal cells with 10 mM NIC resulted in a twofold increase in DNA content and a threefold increase in insulin content. This was associated with the development of beta cell outgrowths from undifferentiated epithelial cell clusters and an increase in the expression of the insulin, glucagon, and somatostatin genes. DNA synthesis was stimulated only in the undifferentiated cells. Half-maximal doses for the insulinotropic and mitogenic effects of NIC were 5-10 and 1-2 mM, respectively. Islet-like cell clusters cultured with NIC responded to glucose stimulation with a biphasic increase in insulin release (fourfold peak), whereas control cells were unresponsive to glucose. Both control and NIC-treated cells developed into functional islet tissue after transplantation into athymic nude mice. As compared with adult islets, the insulinotropic action of NIC could only be demonstrated in the fetal cells. Our results indicate that NIC induces differentiation and maturation of human fetal pancreatic islet cells. This model should be useful for the study of molecular mechanisms involved in beta cell development.
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