Data and Publications
(A) The biological activity of Mouse Recombinant Shh was tested by its ability to promote alkaline phosphatase production of CCL-226
cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.8 μg/mL.
(B) 2 μg of Mouse Recombinant Shh was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant Shh has a predicted molecular mass of 19.8 kDa.