Mitochondrial Tracking Dye, Deep Red

Fluorescent hydrophobic dye that selectively accumulates in mitochondria

Mitochondrial Tracking Dye, Deep Red

Fluorescent hydrophobic dye that selectively accumulates in mitochondria

From: 239 USD
Catalog #
100-0995_C
Fluorescent hydrophobic dye that selectively accumulates in mitochondria

Overview

Efficiently label mitochondria in proliferating and non-proliferating cells with Mitochondrial Tracking Dye, Deep Red. This fixable fluorescent dye uses a quick staining protocol that is well suited for high-throughput screening and is compatible with fluorescence microscopy. Mitochondrial Tracking Dye, Deep Red uses a labeling protocol with minimal hands-on time while promoting photostability and long retention times for enhanced staining efficiency.

Features:
• Excitation wavelength: 640 nm
• Emission wavelength: 659 nm
• Easily crosses live cell membranes due to hydrophobicity
• Partially dependent on mitochondrial membrane potential
• Cell-retaining group produces longer retention times
• Verified for use in fluorescence microscopy

Mitochondrial Tracking Dye, Deep Red is a hydrophobic dye that accumulates in mitochondria and is partially dependent on mitochondrial membrane potential. Upon depolarization, the dye relocalizes to the cytoplasm and becomes non-specific, but still retains its red fluorescence. Mitochondrial tracking dyes contain thiol chloromethyl, a cell-retaining group (Clutton et al. Cytometry A, 2019). The chloromethyl group increases staining efficiency by retaining the fluorescent dye in the mitochondria long after fixation (Clutton et al. Cytometry A, 2019). Due to its vivid far red fluorescence, Mitochondrial Tracking Dye, Deep Red is suitable for multi-labeling experiments such as microplate assays and flow cytometry. Use Mitochondrial Tracking dyes to quantify mitochondrial activity, localization, and abundance.
Application
Flow Cytometry
CAS Number
67-68-5

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0995
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0995
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Figure 1. Human Embryonic Stem Cells Stained with Mitochondrial Tracking Dye, Deep Red

Human embryonic stem cells were cultured using mTesR™ Plus (Catalog #100-0276) on Matrigel®-coated glass-bottom dishes for 24 hours, then labeled with Mitochondrial Tracking Dye, Deep Red (red), and counterstained with Hoechst 33342 (blue).

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