Mitochondrial Tracking Dye, Deep Red
Fluorescent hydrophobic dye that selectively accumulates in mitochondria
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Overview
Features:
• Excitation wavelength: 640 nm
• Emission wavelength: 659 nm
• Easily crosses live cell membranes due to hydrophobicity
• Partially dependent on mitochondrial membrane potential
• Cell-retaining group produces longer retention times
• Verified for use in fluorescence microscopy
Mitochondrial Tracking Dye, Deep Red is a hydrophobic dye that accumulates in mitochondria and is partially dependent on mitochondrial membrane potential. Upon depolarization, the dye relocalizes to the cytoplasm and becomes non-specific, but still retains its red fluorescence. Mitochondrial tracking dyes contain thiol chloromethyl, a cell-retaining group (Clutton et al. Cytometry A, 2019). The chloromethyl group increases staining efficiency by retaining the fluorescent dye in the mitochondria long after fixation (Clutton et al. Cytometry A, 2019). Due to its vivid far red fluorescence, Mitochondrial Tracking Dye, Deep Red is suitable for multi-labeling experiments such as microplate assays and flow cytometry. Use Mitochondrial Tracking dyes to quantify mitochondrial activity, localization, and abundance.
Data Figures

Figure 1. Human Embryonic Stem Cells Stained with Mitochondrial Tracking Dye, Deep Red
Human embryonic stem cells were cultured using mTesR™ Plus (Catalog #100-0276) on Matrigel®-coated glass-bottom dishes for 24 hours, then labeled with Mitochondrial Tracking Dye, Deep Red (red), and counterstained with Hoechst 33342 (blue).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.