Mitochondrial Isolation Kit

Isolation kit to enrich the mitochondrial subcellular fraction from mammalian cells or tissue

Mitochondrial Isolation Kit

Isolation kit to enrich the mitochondrial subcellular fraction from mammalian cells or tissue

From: 285 USD
Catalog #
100-0990_C
Isolation kit to enrich the mitochondrial subcellular fraction from mammalian cells or tissue

What's Included

  • Mitochondrial Isolation Kit (Catalog #100-0990)
    • Isoloation Buffer, 1 x 100 mL
    • Homogenization Reagent, 1 x 1 mL

Overview

Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers a quick mitochondrial isolation technique that has been verified for detection using western blot analysis and may be used to study apoptosis and signaling events between the two fractions.

Features:
• Fast and efficient enrichment of the active mitochondrial subcellular fraction
• Verified for use with western blot analysis

The Mitochondrial Isolation Kit offers two options to isolate the mitochondrial subcellular fraction. The first option is a reagent-based method for processing up to six samples concurrently. The second method utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both methods employ microcentrifugation to separate the mitochondrial fraction from the cytosolic fraction.

The enriched mitochondrial fractions preserve their biological activity and are compatible with downstream applications, including the study of mitochondrial respiration, apoptosis, inner mitochondrial membrane potential, and protein profiling (Itahana & Zhang Cancer Cell, 2008).
Application
Western Blotting

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0990
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0990
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0990
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Figure 1. Western Blot of Mitochondrial Markers After Mitochondrial Isolation from Cultured Cells

Mitochondria were isolated from HEK293 cells with Mitochondrial Isolation Kit using Dounce homogenization. Mitochondrial (M) and cytosolic (C) fractions were analyzed via western blot for two mitochondrial proteins: voltage-dependent anion channels (VDAC) and cytochrome C. Results demonstrate that mitochondria remain intact after enrichment.

Figure 2. Mitochondria Isolated Using the Mitochondrial Isolation Kit Maintain Healthy Mitochondrial Function

Mitochondria were isolated from HEK293 cells using Mitochondrial Isolation Kit and stained with JC-1 (Iodide) dye (Catalog #100-0993) in the presence (grey bar) or absence (orange bar) of valinomycin. High levels of fluorescence indicate that healthy mitochondrial function is retained after isolation.

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