Mitochondrial Isolation Kit
Isolation kit to enrich the mitochondrial subcellular fraction from mammalian cells or tissue
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• Fast and efficient enrichment of the active mitochondrial subcellular fraction
• Verified for use with western blot analysis
The Mitochondrial Isolation Kit offers two options to isolate the mitochondrial subcellular fraction. The first option is a reagent-based method for processing up to six samples concurrently. The second method utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both methods employ microcentrifugation to separate the mitochondrial fraction from the cytosolic fraction.
The enriched mitochondrial fractions preserve their biological activity and are compatible with downstream applications, including the study of mitochondrial respiration, apoptosis, inner mitochondrial membrane potential, and protein profiling (Itahana & Zhang Cancer Cell, 2008).
Figure 1. Western Blot of Mitochondrial Markers After Mitochondrial Isolation from Cultured Cells
Mitochondria were isolated from HEK293 cells with Mitochondrial Isolation Kit using Dounce homogenization. Mitochondrial (M) and cytosolic (C) fractions were analyzed via western blot for two mitochondrial proteins: voltage-dependent anion channels (VDAC) and cytochrome C. Results demonstrate that mitochondria remain intact after enrichment.
Figure 2. Mitochondria Isolated Using the Mitochondrial Isolation Kit Maintain Healthy Mitochondrial Function
Mitochondria were isolated from HEK293 cells using Mitochondrial Isolation Kit and stained with JC-1 (Iodide) dye (Catalog #100-0993) in the presence (grey bar) or absence (orange bar) of valinomycin. High levels of fluorescence indicate that healthy mitochondrial function is retained after isolation.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Mitochondrial Isolation Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.