Live Virtual Training: Human Pulmonary Organoid Culture
Discover protocols and best practices for culturing human pulmonary organoids and their downstream applications.
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In-lab demonstration
Overview
Human pulmonary organoids are emerging as important tools in respiratory research for modeling both the airway and alveolar regions of the lung. Bring these tools and their best practices to your lab by registering for this three-day, part-time course consisting of live-streamed, interactive lectures and laboratory demonstrations. Guided by STEMCELL Technologies’ in-house pulmonary organoid experts, you will learn how to discern which airway culture system is best for your applications as well as gain the skills to successfully build it yourself.
Learning Outcomes:
- Understand the historical context of human pulmonary organoid development
- Describe the progenitors of the pulmonary epithelium
- Understand the functional capabilities of the PneumaCult™ portfolio
- Discern the benefits and drawbacks of air-liquid interface (ALI) culture systems, organoids, and animal models
- Understand the protocols for expanding and differentiating airway cells at the ALI, growing airway organoids in apical-in and apical-out orientations, and generation of alveolar organoids
- Identify key readouts and applications of ALI culture and alveolar organoids
- Choose the correct airway culture system for your application
- Appreciate the expected morphology and marker expression of airway cultures at the ALI, airway organoids, and alveolar organoids
As part of the course, you will receive:
- An electronic copy of the course book
- Certificates of course completion from STEMCELL Technologies, Continuing Professional Development (CPD) UK and PACE
- Post-training support from the trainers and STEMCELL’s Scientific Support team
Space is limited, please apply early for the best chance of securing a seat for your desired date. Registration closes one week before the course date.
Agenda
Day 1 (3 hrs, including introduction, break, and wrap-up/Q&A)
- Introduction (0.25 hr)
- Lecture 1: Introduction to Pulmonary Culture Systems (45 min)
- Lecture 2: Establishing Airway Cultures at the Air-Liquid Interface (45 min)
Day 2 (3.75 hrs, including break and wrap-up/Q&A)
- Lecture 3: Establishing and Assessing Airway Organoids in Two Polarities (45 min)
- Lab Live-Stream: Establishing Airway ALI & Organoid Cultures (2 h)
Day 3 (4.25 hrs, including break and wrap-up/Q&A)
- VLecture 4: Maintaining and Assessing Alveolar Organoids (45 min)
- Lab Live-Stream: Dissociating Alveolar Organoids for Passaging, Cryopreservation & Air-Liquid Interface Culture (1.5 h)
- Guest Lecture: TBD (1 h)
STEMCELL Technologies is approved as a provider of continuing education programs in the clinical laboratory sciences by The American Society for Clinical Laboratory Science (ASCLS) Professional Acknowledgment for Continuing Education (P.A.C.E.®) Program.
This course is certified by the United Kingdom CPD Certification Service, which may be applicable to individuals who are members of, or are associated with, UK-based professional bodies. This course consists of approximately 7.5 hours of learning.
Note: STEMCELL Technologies accept no responsibility, and cannot be held responsible, for the claiming or validation of hours or points.
Policy
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
