Stimulating T cells with JCV (VP1) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This peptide pool is a lyophilized mixture of 86 peptides from major capsid protein VP1 (VP1) of the JC polyomavirus (JCV), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 354 on VP1. VP1 binds LSTc oligosaccharides found on host cell glycoproteins and glycolipids, and this interaction is said to be essential for JCV viral growth and infectivity (Neu et al.). The JCV VP1 (Del Valle et al.) and large T antigen (LT) (Delbue et al.) are detected in JCV-associated pathologies such as progressive multifocal leukoencephalopathy (PML) in patients with HIV. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.