JC-1 (Iodide)

Fluorescent lipophilic dye used to quantify mitochondrial membrane potential and assess cell viability

JC-1 (Iodide)

Fluorescent lipophilic dye used to quantify mitochondrial membrane potential and assess cell viability

From: 319 USD
Catalog #
100-0993_C
Fluorescent lipophilic dye used to quantify mitochondrial membrane potential and assess cell viability

Overview

Distinguish high and low mitochondrial membrane potential in living cells using JC-1 (Iodide) dye. JC-1 offers a fast and convenient staining protocol that is well suited for high-throughput screening and compatible with flow cytometry applications.

Features:
• Excitation wavelength: 515 nm
• Emission wavelength: 530 nm
• Extinction coefficient: 195,000 cm⁻¹M⁻¹
• Fluorescence based on dye concentration
• Verified for use with flow cytometry

JC-1 is a lipophilic, cationic carbocyanine dye. This dye can reversibly change its fluorescence, which depends on the equilibrium between its monomeric and aggregate form. JC-1 exists as a monomer in polarized mitochondria and, upon excitation, it emits a green fluorescence with an emission maximum of 530 nm. At higher concentrations, due to high mitochondrial membrane potential, J-aggregates form and emit a green-orange fluorescence with an emission maximum of 590 nm. JC-1 can be used both qualitatively to measure a shift in fluorescence emission, or quantitatively to detect the difference in fluorescence intensity in channels FL1 (FITC) and FL2 (PE).

The green to orange fluorescent intensity ratio was used to evaluate mitochondrial depolarization occurring in apoptosis (Sivandzade et al. Bio-protocol, 2019). JC-1 has been used to simultaneously evaluate drug resistance parameters P-glycoprotein (P-gp) activity and apoptosis in myeloid leukemia cells (Chaoui et al. Cytometry B Clin Cytom, 2006).
Application
Flow Cytometry
CAS Number
3520-43-2

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
JC-1 (Iodide)
Catalog #
100-0993
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
JC-1 (Iodide)
Catalog #
100-0993
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Figure 1. Mitochondrial Depolarization Is Visualized by a Shift to JC-1 (Iodide) Monomers

Flow cytometry analysis of Jurkat cells labeled with JC-1 (Iodide) after incubation in the presence or absence of staurosporine, an inducer of apoptosis. Mitochondrial depolarization is visualized by the population shift from JC-1 aggregates to JC-1 monomers after treatment with staurosporine.

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