Human Recombinant IFN alpha 1

Interferon alpha 1, His tag

Human Recombinant IFN alpha 1

Interferon alpha 1, His tag

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Overview

Interferon alpha 1 (IFNA1) belongs to the type 1 interferon family of cytokines, which bind interferon alpha receptors (IFNAR; composed of IFNAR1 and IFNAR2 subunits) that are involved in interferon-induced JAK-STAT signaling (Shemesh et al.). Interferons have inhibitory effects on viral replication and pathogenesis, and studies have found that IFN alpha can prevent the spread of herpes simplex virus (HSV) (Mikloska and Cunningham), and human immunodeficiency virus (HIV) (George and Mattapallil). Pegylated forms of IFN alpha are currently approved for the treatment of chronic hepatitis B (Woo et al.). IFN alpha also displays anti-tumor effects by regulating cell growth and proliferation, and it is used in the treatment of different cancers (Tagliaferri et al.). Interferons display alpha-helical structures with four helices forming an antiparallel alpha-helix bundle (Walter). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, interferon alpha 1 from STEMCELL comes lyophilized with ≥ 90% purity, specific activity EC50 ≤ 40-200 pg/mL, and LAL analysis verification ensuring endotoxin levels are ≤ 1.0 EU/μg protein.
Subtype
Cytokines
Alternative Names
IFL, IFN, IFNA, IFNA13, IFN alphaD
Species
Human
Area of Interest
Immunology
Purity
≥ 92%

Data Figures

(A) The biological activity of Human Recombinant IFN alpha 1 was tested by its ability to promote the viability of WISH cells infected with VSV in a cytopathic effect (CPE) assay. Cell viability was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the above example is ≤ 200 pg/mL. (B) Human Recombinant IFN alpha 1 was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant IFN alpha 1 has a predicted molecular mass of 20.8 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1336
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1336
Lot #
All
Language
English