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EasySep™ Release Mouse Biotin Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

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New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.

EasySep™ Release Mouse Biotin Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology

From: 904 USD
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Immunomagnetic positive selection kit using particle release technology
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Product Advantages


  • Highly purified cells labeled with biotinylated antibodies isolated from mouse tissue in less than 40 minutes

  • No-wash removal of EasySep™ Releasable RapidSpheres™

What's Included

  • EasySep™ Release Mouse Biotin Positive Selection Kit (Catalog #17655)
    • EasySep™ Biotin Positive Cocktail, 0.5 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 1 mL
    • EasySep™ Release Buffer (Concentrate), 3 x 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Release Mouse Biotin Positive Selection Kit is designed to isolate cells that are labeled with biotin-conjugated antibodies from mouse tissue by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, biotin-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.

This product replaces the EasySep™ Mouse Biotin Positive Selection Kit (Catalog #18556), providing highly purified particle-free cells.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ Magnet (Catalog #18102)
• EasyEights™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Innate Lymphoid Cells, Lymphocytes, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Data Figures

Starting with fresh PBMCs, the purities of the start and final isolated fractions are 34.6% and 97.1%, respectively, using a biotinylated anti-human CD45RO antibody and EasySep™ Release Human Biotin Positive Selection Kit (as assessed by labeling with CD45RO and CD45RA).

Starting with mouse splenocytes, the purities of the start and final isolated fractions are 58.4% and 95.1%, respectively, using a biotinylated anti-mouse CD19 antibody and EasySep™ Release Mouse Biotin Positive Selection Kit (as assessed by labeling with CD19 and CD45R/B220).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
1000157902 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
1000157901 or lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Release Mouse Biotin Positive Selection Kit
Catalog #
17655
Lot #
All
Language
English

Resources and Publications

Educational Materials (7)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Brochure
EasySep™ Release Free Your Positively Selected Cells from Magnetic Particles
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres™
Scientific Poster
Positive Selection of PE- or Biotin-Conjugated Antibody Labeled Cells with Releasable Rapidspheres�...

Related Products

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Labeling Antibodies

New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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