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EasySep™ Serology Whole Blood CD19 Positive Selection Kit

Immunomagnetic positive selection for HLA serology testing

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EasySep™ Serology Whole Blood CD19 Positive Selection Kit

Immunomagnetic positive selection for HLA serology testing

From: 699 USD
Catalog #
18984_C
Immunomagnetic positive selection for HLA serology testing
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Product Advantages


  • Fast and easy-to-use

  • Up to 98% purity

  • No columns required

  • Compatible with HLA serological applications

What's Included

  • EasySep™ Serology Whole Blood CD19 Positive Selection Kit (Catalog #18984)
    • Anti-Human CD19 Antibody, Biotin, 1 x 80 μL
    • EasySep™ Serology Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50003, 2 x 1 mL
    • EasySep™ Serology Whole Blood CD19 Dissociation Reagent, 3 x 0.5 mL
    • EasySep™ Red Blood Cell Lysis Buffer, 10X Concentrate, 1 x 10 mL
  • RoboSep™ Serology Whole Blood CD19 Positive Selection Kit (Catalog #18984RF)
    • Anti-Human CD19 Antibody, Biotin, 1 x 80 μL
    • EasySep™ Serology Whole Blood CD19 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Streptavidin RapidSpheres™ 50003, 2 x 1 mL
    • EasySep™ Serology Whole Blood CD19 Dissociation Reagent, 3 x 0.5 mL
    • EasySep™ Red Blood Cell Lysis Buffer, 10X Concentrate, 1 x 10 mL
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Tips (Catalog #20125) x 2

Overview

EasySep™ Serology Whole Blood CD19 Positive Selection Kit is designed to isolate highly purified CD19+ cells from fresh human whole blood by immunomagnetic positive selection. This kit targets human CD19+ cells for positive selection with an antibody recognizing the CD19 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as HLA serology, flow cytometry, or DNA/RNA extraction.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
HLA, Immunology

Data Figures

Using the EasySep™ Serology Whole Blood CD19 Positive Selection Kit on human whole blood, the CD19+ purities of the start and final isolated fractions are 7.3% and 96.2%, respectively (gated on CD45+).

Figure 1. EasySep™ Serology Whole Blood CD19 Positive Selection Kit

Starting with human whole blood, the CD19+ cell content of the isolated fraction typically ranges from 89.4 - 97.8% (note: as the CD19 antibody used for flow cytometry staining was fully blocked by the CD19 antibody used to isolate the cells, staining of B cells was assessed by labeling with anti-CD20 antibody; gated on CD45+). In the above example, the purities of the start and final isolated fractions are 7.3% and 96.2%, respectively (gated on CD45+).
NOTE: Red blood cells were removed from start sample by lysis prior to flow cytometry.
NOTE: The magnetic particles present on the surface of the isolated CD19+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer. This will make it difficult to gate on a specific lymphocyte population based on FSC/SSC. Instead, we recommend first gating the cells on CD45 and examining the lymphocyte populations based on the CD45+ gate.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984
Lot #
1000075014 and higher
Language
English
Document Type
Product Information Sheet 2
Product Name
EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984
Lot #
1000065452 and lower
Language
English
Document Type
Product Information Sheet 1
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
1000075014 and higher
Language
English
Document Type
Product Information Sheet 2
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
1000065452 and lower
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
RoboSep™ Serology Whole Blood CD19 Positive Selection Kit
Catalog #
18984RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (8)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Fully Automated Cell Isolation with RoboSep™
Brochure
Fully Automated Cell Isolation with RoboSep™
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
The Human Leukocyte Antigen (HLA) Complex
Wallchart
The Human Leukocyte Antigen (HLA) Complex
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
A New, Automated Solution for Cell Isolation Upstream of Complement-Dependent Cytotoxicity Assay (CDC) Workflow
41:03
Webinar
A New, Automated Solution for Cell Isolation Upstream of Complement-Dependent Cytotoxicity Assay (CD...

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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