EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
Immunomagnetic positive selection cell isolation kit
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EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit -
RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit -
Label for EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
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Required Products
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Cell separation buffer -
"The Big Easy" EasySep™ Magnet
Magnet for column-free immunomagnetic separation
Overview
The EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit is designed to isolate highly purified CD33+ and CD66b+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD33, CD66b and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD33 and CD66b antigens is expressed on myeloid progenitor cells, granulocytes and monocytes.
This product is an improved version of the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) that provides highly purified cells using a shorter protocol.
This product replaces the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA), for even faster cell isolations.
This product is an improved version of the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) that provides highly purified cells using a shorter protocol.
This product replaces the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA), for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99% purity
• No columns required
• Up to 99% purity
• No columns required
Components:
- EasySepSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit (Catalog #17884)
- EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit with Filter Tips (Catalog #17884RF)
- EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Centrifugation Tubes
Cell Type:
Myeloid Cells
Species:
Human
Sample Source:
Whole Blood
Selection Method:
Positive
Brand:
EasySep
Area of Interest:
Chimerism; Immunology
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
17884
All
17884RF
All
17884
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17884
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17884
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17884RF
All
17884RF
All
Educational Materials
(10)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with human whole blood, the myeloid cell content of the isolated fraction is typically 94.5 ± 4.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 66.6% and 98.5%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.