EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit
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EasySep™ BufferCell separation buffer
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"The Big Easy" EasySep™ MagnetMagnet for column-free immunomagnetic separation
Overview
Desired cells are targeted with tetrameric antibody complexes recognizing CD33, CD66b, and dextran-coated magnetic particles, and separated without the use of columns through immunomagnetic positive selection using an EasySep™ magnet. Cells of interest remain in the tube while unwanted cells are poured off.
This product has replaced the EasySep™ Human Whole Blood Myeloid Positive Selection Kit (Catalog #18683) and EasySep™ HLA Whole Blood Myeloid Positive Selection Kit (Catalog #18683HLA) for faster cell isolations. For even higher efficiency, this product can be used with RoboSep™ for automated immunomagnetic cell isolation.
• Up to 99% purity
• No columns required
- EasySepSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit (Catalog #17884)
- EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ HLA Chimerism Whole Blood Myeloid Positive Selection Kit with Filter Tips (Catalog #17884RF)
- EasySep™ HLA Chimerism Whole Blood Myeloid Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ 50101, 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Scientific Resources
Product Documentation
Educational Materials (10)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Starting with human whole blood, the myeloid cell content of the isolated fraction is typically 94.5 ± 4.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 66.6% and 98.5%, respectively.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
