EasySep™ Direct Human Basophil Isolation Kit
Immunomagnetic negative selection from whole blood kit
EasySep™ Direct Human Basophil Isolation Kit
Immunomagnetic negative selection from whole blood kit
Product Advantages
- Effectively depletes RBCs without the need for density gradient centrifugation, sedimentation, or lysis
- Up to 98% purity of isolated cells
- Fast, easy-to-use and column-free
- Isolated cells are untouched
What's Included
- EasySep™ Direct Human Basophil Isolation Kit (Catalog #19667)
- EasySep™ Direct Human Basophil Isolation Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™ 50300, 4 x 2.5 mL
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EasySep™ BufferCell separation buffer
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"The Big Easy" EasySep™ MagnetMagnet for column-free immunomagnetic separation
Overview
Data Figures
Figure 1. Typical EasySep™ Direct Human Basophil Isolation Profile
Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 ± 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (11)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
