EasySep™ Direct Human Basophil Isolation Kit

Immunomagnetic negative selection from whole blood kit

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EasySep™ Direct Human Basophil Isolation Kit

Immunomagnetic isolation of basophils directly from whole blood

100 mL whole blood
Catalog #19667
458 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Direct Human Basophil Isolation Kit isolates functional, highly purified basophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
Advantages:
• Effectively depletes RBCs without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 98% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components:
  • EasySep™ Direct Human Basophil Isolation Kit (Catalog #19667)
    • EasySep™ Direct Human Basophil Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™ 50300, 4 x 2.5 mL
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(11)
Brochure
EasySep™ Direct: Cell Isolation from Whole Blood in 20 Minutes
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
6:13
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasyEights Magnet
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
4:30
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, EasySep™ Magnet (Purple/Silver)
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
1:44
Cell Isolation Directly from Whole Blood without Density Gradient Centrifugation or RBC Lysis: EasySep™ Direct
Video
5:17
How to Isolate Cells Directly from Whole Blood - EasySep™ Direct Protocol, Easy 50 EasySep™ Magnet

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Direct Human Basophil Isolation Profile

Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 ± 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.