MyoCult™

MyoCult™

Media to Expand, Maintain and Differentiate Skeletal Muscle Progenitor Cells (Myoblasts)

MyoCult™ media are specifically formulated to expand, maintain and differentiate primary human myoblasts. These specialized media are designed to provide researchers with standardized workflow and culture systems to minimize cell culture variability and increase experimental reproducibility.

Why Use MyoCult™?

  • Optimized and specifically formulated media to support greater expansion and differentiation of myogenic progenitor cells compared to commercially available media.
  • Rigorous raw material screening and quality control minimize lot-to-lot variability to support optimal and consistent results.
  • MyoCult™ media come in easy-to-use format to save time and streamline myogenic studies.

MyoCult™ Media for Human Skeletal Muscle Progenitor Cells

MyoCult™ Expansion Kit

Species:

Human

Formulation:

Serum-containing

Applications:

  • Expansion and maintenance of primary human skeletal myogenic progenitor cells (myoblasts)
  • Myogenesis research
  • Disease modeling of genetic myopathies (muscular dystrophies)
  • Disease modeling of muscle related cancers (rhabdomyosarcoma)
  • Modeling diseases that affect skeletal muscle (cachexia, diabetes, metabolic disorders)
  • Drug screening platforms
  • Co-culture systems

Features/Advantages:

  • Supports greater expansion of human myogenic progenitor cells than commercially available media
  • Suppresses the expression of myogenic differentiation genes while maintaining the expression of myogenic progenitor genes
  • Rigorous raw material screening and quality control minimize lot-to-lot variability
  • Easy-to-use format with no additional growth factors required
  • Culture-expanded myogenic progenitor cells are compatible with MyoCult™ Differentiation Medium

MyoCult™ Differentiation Kit

Species:

Human

Formulation:

Serum-containing

Applications:

  • In vitro differentiation of human myoblasts into multinucleated myotubes
  • Myogenesis research
  • Disease modeling of genetic myopathies (muscular dystrophies)
  • Disease modeling of muscle related cancers (rhabdomyosarcoma)
  • Modeling diseases that affect skeletal muscle (cachexia, diabetes, metabolic disorders)
  • Drug screening platforms
  • Co-culture systems

Features/Advantages:

  • Supports differentiation of myoblasts to multinucleated myotubes with robust expression of myosin heavy chain
  • Rigorous raw material screening and quality control minimize lot-to-lot variability
  • Attachment substrate is included to support optimal adherence and enhanced myotube morphology
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Data

Figure 1. Enhanced Expansion of Human Myogenic Progenitors (Myoblasts) is Obtained When Using the MyoCult™ Expansion Kit

Myoblasts cultured in MyoCult™ expansion medium show superior expansion rate compared to other commercially available media. Data shown from one representative experiment (n=5).



Figure 2. Human Myogenic Progenitor Cells Expanded in MyoCult™ Expansion Medium Maintain Myoblast Morphology

(A) Small and rounded myoblast morphology were maintained when myogenic progenitors were expanded in MyoCult™ expansion medium. Myoblasts expanded in (B) Commercial Medium 1 or (C) Commercial Medium 2 adopted stringy fibroblast-like characteristics. Images were taken at passage 5.



Figure 3. Myoblasts Expanded using the MyoCult™ Expansion Medium Show Increased Expression of Myogenic Progenitor Transcripts and Reduced Expression of Differentiation Transcripts

Human myogenic progenitors were expanded for 5 passages in MyoCult™ expansion medium or Commercial Medium 1. Total RNA was collected for qRT-PCR analysis of MYF5, PAX3, MYOG, MHC and MCK transcripts. (A) Increased levels of the progenitor markers PAX3 and MYF5 transcripts were observed when myoblasts were expanded using the MyoCult™ expansion medium. (B) Reduced expression of the myogenic differentiation markers MYOG, MHC and MCK transcripts were observed in myoblasts expanded in MyoCult™ expansion medium when compared to myoblasts expanded in Commercial Medium 1. Data expressed as a percent change in mRNA transcript levels compared to cells expanded in Commercial Medium 1.



Figure 4. Human Skeletal Muscle Myogenic Progenitor Cells Cultured in MyoCult™ Expansion Medium are Homogeneous and Maintain Expression of Myogenic Progenitor Cell Surface Markers

Human myoblasts expanded for 5 passages in MyoCult™ expansion medium maintained the expression of typical myogenic progenitor cell surface markers (CD29 and CD56) and lacked the expression of hematopoietic (CD45) and endothelial (CD31) markers.



Figure 5. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts

(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.

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