MyoCult™ Differentiation Kit (Human)
Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes
MyoCult™ Differentiation Kit (Human)
Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes
Product Advantages
- Supports differentiation of myogenic progenitor cells to multinucleated myotubes with strong expression of myosin heavy chain and myogenin markers
- Rigorous raw material screening and quality control minimize lot-to-lot variability
- Attachment substrate is included to support optimal adherence and enhanced myotube morphology
What's Included
- MyoCult™ Differentiation Basal Medium (Human), 190 mL
- MyoCult™ Differentiation 20X Supplement (Human), 10 mL
- ACF Cell Attachment Substrate, 1 mL
Overview
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
Data Figures
Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts
(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (5)
Related Products
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
