MyoCult™ Differentiation Kit (Human)

Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes
MyoCult™ Differentiation Kit (Human)

Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes

200 mL
Catalog # 05965
233 USD
Required Products
  1. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

MyoCult™ Differentiation Kit (Human) includes MyoCult™ Differentiation Medium and Animal Component-Free (ACF) Cell Attachment Substrate. MyoCult™ Differentiation Medium is a serum-based medium specifically formulated for the in vitro differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes. MyoCult™ Differentiation Medium is designed for use with the ACF Cell Attachment Substrate, which has been specifically tested for its ability to support optimal adherence and myotube morphology. This medium is suitable for the differentiation of myogenic progenitor cells previously expanded in serum-containing or serum-free media (e.g. using the MyoCult™-SF Expansion Kit #05980).
Advantages
• Supports differentiation of myogenic progenitor cells to multinucleated myotubes with strong expression of myosin heavy chain and myogenin markers
• Rigorous raw material screening and quality control minimize lot-to-lot variability
• Attachment substrate is included to support optimal adherence and enhanced myotube morphology
Components
  • MyoCult™ Differentiation Basal Medium (Human), 190 mL
  • MyoCult™ Differentiation 20X Supplement (Human), 10 mL
  • ACF Cell Attachment Substrate, 1 mL
Subtype
Specialized Media
Cell Type
Myogenic Stem and Progenitor Cells
Species
Human
Application
Cell Culture, Differentiation
Brand
MyoCult
Area of Interest
Disease Modeling

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
MyoCult™ Differentiation Kit (Human)
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
MyoCult™ Differentiation Kit (Human)
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
MyoCult™ Differentiation Kit (Human)
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
MyoCult™ Differentiation Kit (Human)
Catalog #
05965
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
MyoCult™ Differentiation Kit (Human)
Catalog #
05965
Lot #
All
Language
English

Educational Materials (4)

Brochure
Derive, Expand, and Differentiate Human Skeletal Muscle Progenitor Cells
Wallchart
SnapShot: Exercise Metabolism
Webinar
3D Modeling of Skeletal Muscle for In Vitro Functional Assaying
55:57
3D Modeling of Skeletal Muscle for In Vitro Functional Assaying
Scientific Poster
A Serum-Free Workflow for the Isolation, Expansion and Differentiation of Human Myogenic Progenitors

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts

(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.

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