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Anti-Human IL-4 Antibody, Clone MP4-25D2

Rat monoclonal IgG antibody against human IL-4

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Anti-Human IL-4 Antibody, Clone MP4-25D2

Rat monoclonal IgG antibody against human IL-4

From: 149 USD
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Rat monoclonal IgG antibody against human IL-4
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Overview

This rat monoclonal antibody (clone MP4-25D2) reacts with human interleukin 4 (IL-4). IL-4 is an ~15 kDa protein primarily composed of a four-helix bundle (Powers et al.) and is produced by a host of immune cells including T helper 2 (Th2) cells, basophils, eosinophils, and mast cells (Gadani et al.). It exhibits high affinity for the IL-4 receptor alpha (IL-4Rα) chain, which dimerizes with either the common gamma chain (γc) or IL-13 receptor alpha 1 (IL-13Rα1), leading to activation of downstream JAK-STAT signaling (Heller at al.). IL-4 is involved in the differentiation of CD4+ T lymphocytes into Th2 cells (Silva-Filho et al.), class switching of B cells to IgE (Geha et al.), and activation of M2 macrophages with IL-13 via an alternative pathway (Gordon et al.).
Target Antigen
IL-4
Alternative Names
B cell stimulatory factor 1, BCDF, BCGF, BSF-1, interleukin-4
Reactive Species
Human
Conjugation
APC, PE, Unconjugated
Host Species
Rat
Cell Type
T Cells, CD4+
Species
Human
Application
Flow Cytometry
Area of Interest
Immunology
Clone
MP4-25D2
Gene ID
3565
Isotype
IgG1, kappa

Data Figures

Flow Cytometry Analysis Of Unstimulated And Stimulated Human PBMCs Labeled With Anti-Human IL-4 Antibody, Clone MP4-25D2

Figure 1. Data for Anti-Human IL-4 Antibody, Clone MP4-25D2, PE

(A) Flow cytometry analysis of unstimulated human peripheral blood mononuclear cells (PBMCs) labeled with anti-human IL-4 Antibody, Clone MP4-25D2, PE and anti-human CD8a antibody, clone RPA-T8, APC (Catalog #60022AZ.1). (B) Flow cytometry analysis of PMA/ionomycin-stimulated human peripheral blood mononuclear cells (PBMCs) labeled with anti-human IL-4 Antibody, Clone MP4-25D2, PE and anti-human CD8a antibody, clone RPA-T8, APC (Catalog #60022AZ.1).

Flow Cytometry Analysis Of Unstimulated And Stimulated Human PBMCs Labeled With Anti-Human CD8a Antibody, Clone RPA-T8, PE

Figure 2. Data for Anti-Human IL-4 Antibody, Clone MP4-25D2, APC

(A) Flow cytometry analysis of unstimulated human peripheral blood mononuclear cells (PBMCs) labeled with anti-human IL-4 Antibody, Clone MP4-25D2, APC and anti-human CD8a antibody, clone RPA-T8, PE (Catalog #60022PE). (B) Flow cytometry analysis of PMA/ionomycin-stimulated human peripheral blood mononuclear cells (PBMCs) labeled with anti-human IL-4 Antibody, Clone MP4-25D2, APC and anti-human CD8a antibody, clone RPA-T8, PE (Catalog #60022PE).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human IL-4 Antibody, Clone MP4-25D2, PE
Catalog #
100-1452
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human IL-4 Antibody, Clone MP4-25D2
Catalog #
100-1454
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human IL-4 Antibody, Clone MP4-25D2, APC
Catalog #
100-1453
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human IL-4 Antibody, Clone MP4-25D2, PE
Catalog #
100-1452
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human IL-4 Antibody, Clone MP4-25D2
Catalog #
100-1454
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human IL-4 Antibody, Clone MP4-25D2, APC
Catalog #
100-1453
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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