This mouse monoclonal antibody (clone 1-D1K) reacts with human interferon-gamma (IFN-γ). IFN-γ is an ~17 kDa protein produced in response to mitogenic and antigenic stimuli by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ is controlled by cytokines such as IL-12 and IL-18 secreted in response to infection (Schroder et al.). IFN-γ functions as a homodimer that is composed of interconnecting alpha-helices (Thiel et al.), which bind to the alpha subunit of the IFN-γ receptor (IFNGR) on target cells, resulting in receptor crosslinking and activation of JAK-STAT signaling (Kak et al.). IFN-γ mediates many cellular effects, including upregulation of class I and II antigen presentation pathways, antiviral and antimicrobial mechanisms, antiproliferative and apoptotic effects, immunomodulation, and leukocyte trafficking (Schroder et al.).