This rabbit polyclonal antibody reacts with human, mouse, and rat cytokeratin 20, a low molecular weight, type I cytokeratin that is a major component of mature enterocytes and goblet cells. Cytokeratin 20 is expressed primarily in the gastrointestinal (GI) epithelium, urothelium, and Merkel cells and serves as a marker of GI differentiation. Use this polyclonal antibody in applications requiring detection of cytokeratin 20, such as western blotting (WB) assays, immunohistochemistry protocols (IHC-P), or immunofluorescence (IF). It is verified for use with immunocytochemistry (ICC).

The cytokeratin 20 protein is responsible for the structural integrity of epithelial cells and maintains the intermediate filament organization in the intestinal epithelia. Cytokeratin 20 is also a useful marker of pancreatic and colorectal cancer and has been detected in adenocarcinomas of the colon, pancreas, stomach and biliary tract. Expression of cytokeratin 20 has also been associated with the progression and metastasis of breast cancer, suggesting that it may be a biomarker in predicting breast cancer prognosis.