Anti-Human CD24 Antibody, Clone 32D12

Mouse monoclonal IgG1 antibody against human CD24

Anti-Human CD24 Antibody, Clone 32D12

Mouse monoclonal IgG1 antibody against human CD24

From: 223 USD
Catalog #
60134_C
Mouse monoclonal IgG1 antibody against human CD24

Overview

The 32D12 antibody reacts with CD24, a 35 - 50 kDa glycosylphosphatidylinositol (GPI)-linked sialoglycoprotein that is anchored to the plasma membrane and is glycosylated variably depending on cell type. The function of CD24 has yet to be fully determined but it is believed to play a role in lymphocyte development, particularly in acting as a costimulatory molecule for CD4+ T cells and in regulating B cell proliferation, activation, differentiation, and apoptosis. CD24 may also function in cell adhesion and metastasis, likely through binding of CD62P (P-Selectin). CD24 is expressed by follicular dendritic cells, and various epithelial and hematopoietic cell types, including granulocytes, erythrocytes, most B cells, and subsets of T lymphocytes. As it is expressed throughout B cell development but is lost after differentiation into the plasma cell stage, CD24 may be used as a B cell differentiation marker. Furthermore, CD24 also has the potential to be a marker for tumor prognosis and diagnosis as it is highly expressed in numerous cancers including ovarian, breast, prostate, bladder, renal, and lung, as well as neuroblastomas and various hematological malignancies. Reportedly, the 32D12 antibody recognizes an epitope that is distinct from those recognized by clones VIB-C5, VIB-E3, LC66, BA-1, ML5, OKB2 and SN3 A5-2H10.
Subtype
Primary Antibodies
Target Antigen
CD24
Alternative Names
CD24a, Heat stable antigen, HSA, Ly-52, Nectadrin, Small cell lung carcinoma cluster 4 antigen
Reactive Species
Human
Conjugation
Biotin, FITC
Host Species
Mouse
Cell Type
B Cells
Species
Human
Application
Cell Isolation, Flow Cytometry
Area of Interest
Immunology
Clone
32D12
Gene ID
100133941
Isotype
IgG1, kappa

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60134
Lot #
All
Language
English
Catalog #
60134BT
Lot #
All
Language
English
Catalog #
60134FI
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
60134
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
60134
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60134BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60134FI
Lot #
All
Language
English

Data and Publications

Figure 1. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD24 Antibody, Clone 32D12 followed by a rat anti-mouse IgG1 antibody, FITC (filled histogram), or a mouse IgG1, kappa isotype control antibody followed by a rat anti-mouse IgG1 antibody, FITC (solid line histogram).

Figure 2. Data for Biotin-conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD24 Antibody, Clone 32D12, Biotin followed by streptavidin (SAV) FITC (filled histogram), or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60070BT) followed by SAV FITC (solid line histogram).

Figure 3. Data for FITC-conjugated

Flow cytometry analysis of human perifpheral blood mononuclear cells (PBMCs; gated on lymphocytes) labeled with Anti-Human CD24 Antibody, Clone 32D12, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

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