Mitochondrial Tracking Dye, Blue

Fluorescent hydrophobic dye that selectively accumulates in mitochondria

Mitochondrial Tracking Dye, Blue

Fluorescent hydrophobic dye that selectively accumulates in mitochondria

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Fluorescent hydrophobic dye that selectively accumulates in mitochondria
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Overview

Rapidly label mitochondria in proliferating and non-proliferating cells with Mitochondrial Tracking Dye, Blue. This fixable fluorescent dye uses a quick and simple staining protocol that is well suited for high-throughput screening and is compatible with fluorescence microscopy. Mitochondrial Tracking Dye, Blue uses a labeling protocol with minimal hands-on time while promoting photostability and long retention times for enhanced staining efficiency.

Features:
• Excitation wavelength: 344 nm
• Emission wavelength: 469 nm
• Easily crosses live cell membranes due to hydrophobicity
• Partially dependent on mitochondrial membrane potential
• Cell-retaining group produces longer retention times

Mitochondrial Tracking Dye, Blue is a hydrophobic dye that accumulates in mitochondria and is partially dependent on mitochondrial membrane potential. Upon depolarization, the dye relocalizes to the cytoplasm and becomes non-specific, but still retains its blue fluorescence. This mitochondrial tracking dye contains a cell-retaining group—thiol chloromethyl—that increases staining efficiency by retaining fluorescence long after fixation (Clutton et al. Cytometry A, 2019). Mitochondrial Tracking Dye, Blue is suitable for multi-labeling experiments such as microplate assays and flow cytometry. Use mitochondrial tracking dyes to quantify mitochondrial activity, localization, and abundance.
Application
Flow Cytometry
CAS Number
67-68-5
Molecular Weight
~600 g/mol

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0996
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0996
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.