MyoCult™ Differentiation Kit (Human)
Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes
MyoCult™ Differentiation Kit (Human)
Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes
Product Advantages
• Rigorous raw material screening and quality control minimize lot-to-lot variability
• Attachment substrate is included to support optimal adherence and enhanced myotube morphology
What's Included
- MyoCult™ Differentiation Basal Medium (Human), 190 mL
- MyoCult™ Differentiation 20X Supplement (Human), 10 mL
- ACF Cell Attachment Substrate, 1 mL
-
D-PBS (Without Ca++ and Mg++)Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
Related Products
Scientific Resources
Product Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Educational Materials (4)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts
(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
