MyoCult™ Differentiation Kit (Human)
Medium for the differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes
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MyoCult™ Differentiation Kit (Human) -
Label for MyoCult™ Differentiation Kit (Human) -
Label for MyoCult™ Differentiation Kit (Human) -
Label for MyoCult™ Differentiation Kit (Human)
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Required Products
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Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
MyoCult™ Differentiation Kit (Human) includes MyoCult™ Differentiation Medium and Animal Component-Free (ACF) Cell Attachment Substrate. MyoCult™ Differentiation Medium is a serum-based medium specifically formulated for the in vitro differentiation of human skeletal muscle progenitor cells (myoblasts) into myotubes. MyoCult™ Differentiation Medium is designed for use with the ACF Cell Attachment Substrate, which has been specifically tested for its ability to support optimal adherence and myotube morphology. This medium is suitable for the differentiation of myogenic progenitor cells previously expanded in serum-containing or serum-free media (e.g. using the MyoCult™-SF Expansion Kit #05980).
Advantages:
•Supports differentiation of myogenic progenitor cells to multinucleated myotubes with strong expression of myosin heavy chain and myogenin markers
• Rigorous raw material screening and quality control minimize lot-to-lot variability
•Attachment substrate is included to support optimal adherence and enhanced myotube morphology
• Rigorous raw material screening and quality control minimize lot-to-lot variability
•Attachment substrate is included to support optimal adherence and enhanced myotube morphology
Components:
- MyoCult™ Differentiation Basal Medium (Human), 190 mL
- MyoCult™ Differentiation 20X Supplement (Human), 10 mL
- ACF Cell Attachment Substrate, 1 mL
Subtype:
Specialized Media
Cell Type:
Myogenic Stem and Progenitor Cells
Species:
Human
Application:
Cell Culture; Differentiation
Brand:
MyoCult
Area of Interest:
Disease Modeling
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MyoCult™-SF Expansion Supplement Kit (Human)
Serum-free supplement and attachment substrate for the derivation and expansion of human skeletal muscle progenitor cells (myoblasts) -
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Immunomagnetic positive selection cell isolation kit -
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Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Figure 1. MyoCult™ Differentiation Medium Induces Robust Differentiation of Myotubes from Human Myoblasts
(A) Myogenic progenitor cells expanded in MyoCult expansion medium expressed MyoD (green) and desmin (red). (B) Robust differentiation of myoblasts (shown in (A)) to multinucleated myotubes expressing myosin heavy chain (MyHC+) was achieved following culture in MyoCult™ Differentiation Medium for as little as 5 days. (C) A fusion index showing approximately 75% of total nuclei were found within myotubes expressing MyHC.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.