ImmunoCult™-ACF Human B Cell Expansion Supplement

Serum-free and animal component-free culture supplement for expansion of human B cells
ImmunoCult™-ACF Human B Cell Expansion Supplement

Serum-free and animal component-free culture supplement for expansion of human B cells

2 mL
Catalog # 10974
949 USD
Required Products
  1. ImmunoCult™-XF T Cell Expansion Medium, 500 mL
    ImmunoCult™-XF T Cell Expansion Medium

    Serum-free and xeno-free medium for the expansion of human T cells

Overview

ImmunoCult™-ACF Human B Cell Expansion Supplement is a serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.

ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) to prepare a complete serum-free medium for culturing and expanding human B cells.
Advantages
⦁ Robust in vitro expansion of human B cells without the use of serum, feeder cells, or specialized culture plates
⦁ Serum-free and animal component-free supplement
Contains
Recombinant proteins, including CD40L and other cytokines

Chemically defined components
Subtype
Supplements
Cell Type
B Cells
Species
Human
Application
Activation, Cell Culture, Differentiation, Expansion, Genome Editing
Brand
ImmunoCult
Area of Interest
Antibody Development, Immunology, Infectious Diseases
Formulation
Animal Component-Free, Serum-Free

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English

Educational Materials(6)

Brochure
Isolate Human Immune Cells
Wallchart
Antigen Processing and Presentation
Wallchart
The Immune Response to HIV Poster
Webinar
34:58
Online Immunology Journal Club: The Potential of Antibody-Producing B Cells for Modelling and Therapy
Webinar
28:44
Online Immunology Journal Club: Advances in Live-Attenuated Vaccine Development for Zika Virus
Scientific Poster
Robust Serum-Free Expansion of Human B Cells In Vitro with an Animal Component-Free Cell Culture Supplement

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Fold expansion and maturation of B cells cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement

Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™-ACF Human B Cell Expansion Supplement

B cells isolated from human peripheral blood mononuclear cells (PBMCs) (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1,190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.

Microscopy image of human B cells expanded in vitro without the use of serum, feeder cells, or specialized culture plates

Figure 2. Light Microscopy Image of Cultured Human B Cells

B cells isolated from human PBMCs (Leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged on day 4 after seeding and imaged at 40x magnification on day 6.

Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement

Memory B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each time point. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Figure 4. Expansion and Viability of Human Naive B Cells Cultured with ImmunoCult™-ACF Human B Cell Expansion Supplement

Naive B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement. ImmunoCult™-XF T Cell Expansion Medium was used as a base medium. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each time point. Data represent the mean ± 1 SD of triplicate cultures for the same donor.

Figure 5. Expansion of Human Pan-B Cells Cultured with Various Base Media Supplemented with ImmunoCult™-ACF Human B Cell Expansion Supplement or 10% FBS

Pan-B cells were isolated from human PBMCs (Leukopak) using EasySep™ Human Pan-B Cell Isolation Kit and seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with various base media supplemented with either ImmunoCult™-ACF Human B Cell Expansion Supplement or 10% FBS. The cells were passaged every 3 - 4 days and the fold expansion of viable cells was calculated at each time point. Data represent the mean + 1SD for n = 3 donors (each culture condition was performed in triplicate).

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