EasySep™ Rat B Cell Isolation Kit
Immunomagnetic negative selection kit
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Required Products
-
EasySep™ MagnetMagnet for column-free immunomagnetic separation
-
EasySep™ BufferCell separation buffer
Overview
The EasySep™ Rat B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 95% purity
• Isolated cells are untouched
• Up to 95% purity
• Isolated cells are untouched
Components
EasySep™ Rat B Cell Isolation Kit (Catalog #19644)
- EasySep™ Rat B Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Rat B Cell Isolation Kit (Catalog #19644RF)
- EasySep™ Rat B Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50102, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• ”The BigEasy” EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Other, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
Related Products
Related Products
Scientific Resources
Product Documentation
| Document Type | Product Name | Catalog # | Lot # | Language |
|---|---|---|---|---|
|
Document Type
Product Information Sheet
|
Product Name
EasySep™ Rat B Cell Isolation Kit
|
Catalog #
19644 |
Lot #
All |
Language
English |
|
Document Type
Product Information Sheet
|
Product Name
RoboSep™ Rat B Cell Isolation Kit
|
Catalog #
19644RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
EasySep™ Rat B Cell Isolation Kit
|
Catalog #
19644 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
EasySep™ Rat B Cell Isolation Kit
|
Catalog #
19644 |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 1
|
Product Name
RoboSep™ Rat B Cell Isolation Kit
|
Catalog #
19644RF |
Lot #
All |
Language
English |
|
Document Type
Safety Data Sheet 2
|
Product Name
RoboSep™ Rat B Cell Isolation Kit
|
Catalog #
19644RF |
Lot #
All |
Language
English |
Educational Materials (8)
Brochure
EasySep™ Cell Separation Technology
Brochure
Fully Automated Cell Isolation with RoboSep™
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Immune Cells in Rat Tissue
Video
1:23
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Data and Publications
Data
Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
